钙在肿瘤促进剂诱导的缝隙连接细胞间通讯抑制中的作用。

The role of calcium in the tumor promoter-induced inhibition of gap junctional intercellular communication.

机构信息

Agrotechnological Research Institute (ATO-DLO), Department of Food Safety, P.O.Box 17, 6700 AA Wageningen, The Netherlands; Agricultural University Wageningen, Department of Toxicology, Tuinlaan 5, 6703 HE Wageningen, The Netherlands.

出版信息

Environ Toxicol Pharmacol. 1997 Feb 15;3(1):13-6. doi: 10.1016/s1382-6689(96)00132-9.

DOI:10.1016/s1382-6689(96)00132-9

PMID:21781752

Abstract

The effect of several tumor promoters (12-O-tetradecanoyl-phorbol-13-acetate (TPA); 1,1'-(2,2,2-trichloroethylidene)bis[4-chlorobenzene] (DDT); Aroclor1260, and clofibrate) on the inhibition of gap junctional intercellular communication (GJIC) and intracellular calcium concentration (Ca(2+)) was studied in a cell line consisting of initiated cells (3PC). In addition, the effect of different extracellular calcium concentrations (Ca(2+)) on the effects of tumor promoters on both GJIC and Ca(2+) were studied. Agents with GJIC inhibiting capacity increased Ca(2+). However, the increase of Ca(2+) did not (always) precede GJIC inhibition. The effect of tumor promoters on GJIC were similar under low (0.05 mM) and high (1.20 mM) Ca(2+)(e) conditions, while different effects on Ca(2+) were found. These results suggest that tumor promoters can inhibit GJIC and change Ca(2+), but that there is no direct relationship between these two processes.

摘要

几种肿瘤促进剂（12-O-十四烷酰佛波醇-13-醋酸酯（TPA）；1,1'-(2,2,2-三氯乙基)双[4-氯苯]（DDT）；Aroclor1260 和氯贝酸）对起始细胞（3PC）组成的细胞系中细胞间隙连接细胞间通讯（GJIC）和细胞内钙离子浓度(Ca(2+))的抑制作用进行了研究。此外，还研究了不同细胞外钙离子浓度(Ca(2+))对肿瘤促进剂对 GJIC 和 Ca(2+)的影响。具有 GJIC 抑制能力的试剂会增加 Ca(2+)。然而，Ca(2+)的增加并不总是先于 GJIC 抑制。在低（0.05 mM）和高（1.20 mM）Ca(2+)条件下，肿瘤促进剂对 GJIC 的作用相似，而对 Ca(2+)的作用则不同。这些结果表明，肿瘤促进剂可以抑制 GJIC 并改变 Ca(2+)，但这两个过程之间没有直接关系。