豚鼠细胞介导的肿瘤免疫：铬释放试验可检测同基因化学诱导肿瘤的细胞溶解和血清阻断作用。

Guinea pig cell-mediated tumor immunity: the chromium release assay detects both cytolysis and serum blocking for syngeneic chemically-induced tumors.

作者信息

Miller F R, Blazkovec A A

出版信息

Immunol Commun. 1979;8(1):31-47. doi: 10.3109/08820137909044704.

DOI:10.3109/08820137909044704

Abstract

The chromium release test (CRT) was used to assess cell-mediated immunity to syngeneic, chemically-induced tumors in guinea pigs. The animal models were Sewall Wright strain 13 guinea pigs with 3-methyl-cholanthrene (MCA)-induced fibrosarcomas and strain 2 guinea pigs with diethylnitrosamine (DEN)-induced hepatocarcinomas. Regional lymph node cells were significantly cytolytic for the immunizing tumors, specifically so for three of the four tumors, and tumor-bearer sera could significantly block cytolysis. The two DEN-induced strain 2 hepatomas, line 1 and line 10, are antigenically distinct by the CRT but the two MCA-induced tumors have tumor specific antigens as well as a common, cross-reactive antigen.

摘要

采用铬释放试验（CRT）评估豚鼠对同基因化学诱导肿瘤的细胞介导免疫。动物模型为塞瓦尔·赖特13品系豚鼠，其患有3-甲基胆蒽（MCA）诱导的纤维肉瘤，以及2品系豚鼠，其患有二乙基亚硝胺（DEN）诱导的肝癌。区域淋巴结细胞对免疫肿瘤具有显著的细胞溶解作用，对四种肿瘤中的三种尤为明显，且荷瘤动物血清可显著阻断细胞溶解。两种DEN诱导的2品系肝癌，1号线和10号线，通过CRT在抗原性上不同，但两种MCA诱导的肿瘤既有肿瘤特异性抗原，也有一种共同的交叉反应抗原。