Laboratory of Animal Development Biology, College of Life Science, Northeast Forestry University, Harbin, China.
FEBS Lett. 2011 Aug 19;585(16):2592-8. doi: 10.1016/j.febslet.2011.06.039. Epub 2011 Jul 20.
MiR-17-92 cluster miRNAs are disclosed to contribute to the development of multiple organs and tumorigenesis, but their roles in pancreas development remains unclear. In this study, we found that miR-19b, a member of miR-17-92, was highly expressed in the pancreatic progenitor cells, and miR-19b could target the 3' UTR of NeuroD1 mRNA to decrease its protein and mRNA levels. Functional analysis showed that miR-19b exerted little effect on the proliferation of pancreatic progenitors, whereas it inhibited the expression of insulin 1, but not insulin 2 in MIN6 cells. These results suggest that miR-19b can downregulate insulin 1 expression through targeting transcription factor NeuroD1, and thus regulate the differentiation and function of β-cells.
miR-17-92 簇 miRNA 被揭示有助于多种器官的发育和肿瘤发生，但它们在胰腺发育中的作用尚不清楚。在这项研究中，我们发现 miR-19b，miR-17-92 的一个成员，在胰腺祖细胞中高度表达，miR-19b 可以靶向 NeuroD1 mRNA 的 3'UTR，降低其蛋白和 mRNA 水平。功能分析表明，miR-19b 对胰腺祖细胞的增殖影响不大，而它可以抑制 MIN6 细胞中胰岛素 1 的表达，但不能抑制胰岛素 2 的表达。这些结果表明，miR-19b 可以通过靶向转录因子 NeuroD1 下调胰岛素 1 的表达，从而调节 β 细胞的分化和功能。
