Ranera Beatriz, Lyahyai Jaber, Romero Antonio, Vázquez Francisco José, Remacha Ana Rosa, Bernal María Luisa, Zaragoza Pilar, Rodellar Clementina, Martín-Burriel Inmaculada
Laboratorio de Genética Bioquímica (LAGENBIO), Facultad de Veterinaria, Universidad de Zaragoza, 50013, Zaragoza, Spain.
Vet Immunol Immunopathol. 2011 Nov 15;144(1-2):147-54. doi: 10.1016/j.vetimm.2011.06.033. Epub 2011 Jul 2.
Bone marrow and adipose tissue are the two main sources of mesenchymal stem cell (MSC). The aim of this work was to analyse the immunophenotype of 7 surface markers and the expression of a panel of 13 genes coding for cell surface markers in equine bone marrow and adipose tissue-derived MSCs obtained from 9 horses at third passage. The tri-lineage differentiation was confirmed by specific staining. Equine MSCs from both sources were positive for the MSC markers CD29 and CD90, while were negative for CD44, CD73, CD105, CD45 and CD34. The gene expression of these molecules was also evaluated by reverse transcriptase real-time quantitative PCR along with the expression of 5 other MSC markers. Both populations of cells expressed CD13, CD29, CD44, CD49d, CD73, CD90, CD105, CD106, CD146 and CD166 transcripts. Significant differences in gene expression levels between BM- and AT-MSCs were observed for CD44, CD90, CD29 and CD34. Both cell types were negative for CD45 and CD31. The surface antigens tested revealed a similar phenotypic profile between horse and human MSCs, although specific differences in some surface antigens were noticed.
骨髓和脂肪组织是间充质干细胞（MSC）的两个主要来源。本研究的目的是分析从9匹马的第三代骨髓和脂肪组织来源的间充质干细胞中7种表面标志物的免疫表型以及一组编码细胞表面标志物的13个基因的表达情况。通过特异性染色证实了三系分化。两种来源的马间充质干细胞对间充质干细胞标志物CD29和CD90呈阳性，而对CD44、CD73、CD105、CD45和CD34呈阴性。这些分子的基因表达也通过逆转录实时定量PCR以及其他5种间充质干细胞标志物的表达进行了评估。两种细胞群体均表达CD13、CD29、CD44、CD49d、CD73、CD90、CD105、CD106、CD146和CD166转录本。在CD44、CD90、CD29和CD34方面，观察到骨髓来源的间充质干细胞（BM-MSCs）和脂肪组织来源的间充质干细胞（AT-MSCs）之间基因表达水平存在显著差异。两种细胞类型对CD45和CD31均呈阴性。所检测的表面抗原显示马和人间充质干细胞之间具有相似的表型特征，尽管在一些表面抗原上存在特定差异。
