Instituto de Salud Tropical, Departamento de Microbiología y Parasitología, Universidad de Navarra, 31008, Pamplona, Spain.
Vet Microbiol. 2011 Dec 29;154(1-2):152-5. doi: 10.1016/j.vetmic.2011.06.035. Epub 2011 Jul 2.
Rapid and specific identification of Brucella suis at the biovar level is necessary because some of the biovars that infect animals are pathogenic for humans. None of the molecular typing methods described so far are able to discriminate B. suis biovars in a single test and differentiation of B. suis from Brucella canis by molecular approaches can be difficult. This article describes a new multiplex PCR assay, Suis-ladder, for fast and accurate identification of B. suis at the biovar level and the differentiation of B. suis, B. canis and Brucella microti. An advancement of the original Bruce-ladder PCR protocol which allows the correct discrimination of all known Brucella species is also described.
快速且特异性地鉴定猪种布鲁氏菌的生物型是必要的，因为感染动物的某些生物型对人类具有致病性。到目前为止，描述的所有分子分型方法都不能在单次试验中区分猪种布鲁氏菌的生物型，并且通过分子方法区分猪种布鲁氏菌和犬种布鲁氏菌可能很困难。本文描述了一种新的多重 PCR 检测方法，即 Suis-ladder，用于快速准确地鉴定猪种布鲁氏菌的生物型，并区分猪种布鲁氏菌、犬种布鲁氏菌和微小种布鲁氏菌。还描述了原始 Bruce-ladder PCR 方案的改进版本，该版本可正确区分所有已知的布鲁氏菌属物种。
