Departamento de Ingeniería Eléctrica, Electrónica, Automática y Comunicaciones, ETSI Industriales, Universidad de Castilla La Mancha, 13071 Ciudad Real, Spain.
Colloids Surf B Biointerfaces. 2011 Nov 1;88(1):191-5. doi: 10.1016/j.colsurfb.2011.06.030. Epub 2011 Jun 29.
The application of oxidized chromium as a reusable platform for the development of immunosensors is presented. Chromium films were deposited on quartz crystal microbalances to study the affinity interaction between rabbit immunoglobulin G (IgG) and goat anti-rabbit IgG. A covalent approach, based on the silane chemistry, was followed for the grafting of either the rabbit IgG or the anti-rabbit IgG on the silane-modified chromium surface. Next the differences between the deposition of rabbit IgG on immobilized anti-rabbit IgG and the deposition of anti-rabbit IgG on immobilized rabbit IgG were investigated. The chromium layer could be reused between experiments, after the proper removal of the organic layers with piranha etch, obtaining a high repeatability in the steps of the functionalization protocol.
介绍了氧化铬作为可重复使用的免疫传感器开发平台的应用。在石英晶体微天平上沉积铬膜，以研究兔免疫球蛋白 G（IgG）与山羊抗兔 IgG 之间的亲和相互作用。采用基于硅烷化学的共价方法，将兔 IgG 或抗兔 IgG 接枝到硅烷修饰的铬表面上。然后研究了兔 IgG 在固定化抗兔 IgG 上的沉积与抗兔 IgG 在固定化兔 IgG 上的沉积之间的差异。通过用重铬酸和硫酸的混合物（即“piranha 溶液”）适当去除有机层，铬层可以在实验之间重复使用，从而在功能化方案的各个步骤中获得高重复性。
