Department of Pharmaceutical Sciences, College of Pharmacy, North Dakota State University, Fargo, ND 58105, USA.
Environ Toxicol Pharmacol. 2001 Jul;10(3):123-31. doi: 10.1016/s1382-6689(01)00092-8.
The effect of jet propellant-8 (JP-8) fuel exposure on the biophysical, macroscopic and microscopic changes in vitro in porcine skin has been investigated. Fourier transform infrared (FTIR) spectroscopy was employed to investigate the biophysical changes in stratum corneum (SC) lipid and protein. FTIR results showed that the treatment of the SC with JP-8 to increasing exposure time caused correspondingly greater percent decrease in the peak heights and areas under the absorbance curve of methylene and amide absorbances, suggesting greater loss of lipid and protein from SC layers. In vitro transepidermal water loss (TEWL) studies allowed an investigation into the macroscopic barrier properties of the skin. TEWL results were in consonance with that of FTIR. There was a significant increase (P<0.05) in TEWL through 8 and 24 h JP-8 exposed skin in comparison to the control. Light microscopy provided direct, corroborative, visual evidences of epidermal and dermal alterations. Epidermal swelling, dermal matrix granulation, mast cell granules, shortened collagen fibers were observed in the skin exposed with JP-8. Thus, it is concluded that JP-8 exposure causes appreciable biophysical and histological changes along with increased TEWL values in vitro in pig skin which may lead to skin irritation and dermal toxicity in vivo.
已经研究了喷气推进剂-8（JP-8）燃料暴露对体外猪皮生物物理、宏观和微观变化的影响。傅里叶变换红外（FTIR）光谱用于研究角质层（SC）脂质和蛋白质的生物物理变化。FTIR 结果表明，SC 用 JP-8 处理以增加暴露时间会导致相应的亚甲基和酰胺吸收峰高度和吸收曲线下面积的百分比下降更大，表明 SC 层中脂质和蛋白质的损失更大。体外经皮水分流失（TEWL）研究允许研究皮肤的宏观屏障特性。TEWL 结果与 FTIR 的结果一致。与对照相比，8 小时和 24 小时 JP-8 暴露皮肤的 TEWL 显著增加（P<0.05）。光学显微镜提供了表皮和真皮改变的直接、佐证、直观证据。在暴露于 JP-8 的皮肤中观察到表皮肿胀、真皮基质颗粒、肥大细胞颗粒、缩短的胶原纤维。因此，得出结论，JP-8 暴露会导致体外猪皮的生物物理和组织学变化以及 TEWL 值的增加，这可能导致体内皮肤刺激和皮肤毒性。
