Department of Pharmaceutical Sciences, College of Pharmacy, North Dakota State University, Fargo, ND 58105, USA.
Environ Toxicol Pharmacol. 2003 Jun;14(1-2):77-85. doi: 10.1016/S1382-6689(03)00028-0.
JP-8 has been associated with toxicity in animal models and humans. There is a great potential for human exposure to JP-8. Quantitation of percutaneous absorption of JP-8 is necessary for assessment of health hazards involved in its occupational exposure. In this study, we selected three aliphatic (dodecane, tridecane, and tetradecane) and two aromatic (naphthalene and 2-methylnaphthalene) chemicals, which are major components of JP-8. We investigated the changes in skin lipid and protein biophysics, and macroscopic barrier perturbation from dermal exposure of the above five chemicals. Fourier transform infrared (FTIR) spectroscopy was employed to investigate the biophysical changes in stratum corneum (SC) lipid and protein. FTIR results showed that all of the above five components of JP-8 significantly (P<0.05) extracted SC lipid and protein. Macroscopic barrier perturbation was determined by measuring the rate of transepidermal water loss (TEWL). All of the five JP-8 components studied, caused significant (P<0.05) increase in TEWL in comparison to control. We quantified the amount of chemicals absorbed assuming 0.25 m(2) body surface area exposed for 8 h. Our findings suggest that tridecane exhibits greater permeability through skin among aliphatic and naphthalene among aromatic JP-8 components. Amount of chemicals absorbed suggests that tridecane, naphthalene and its methyl derivatives should be monitored for their possible systemic toxicity.
JP-8 已被证实存在于动物模型和人类的毒性。人类接触 JP-8 的可能性很大。定量测定 JP-8 的经皮吸收对于评估其职业暴露所涉及的健康危害是必要的。在这项研究中，我们选择了三种脂肪族（十二烷、十三烷和十四烷）和两种芳香族（萘和 2-甲基萘）化学物质，它们是 JP-8 的主要成分。我们研究了五种化学物质经皮暴露后皮肤脂质和蛋白质生物物理学以及宏观屏障干扰的变化。傅里叶变换红外（FTIR）光谱用于研究角质层（SC）脂质和蛋白质的生物物理变化。FTIR 结果表明，JP-8 的上述五种成分都显著（P<0.05）提取了 SC 脂质和蛋白质。宏观屏障干扰通过测量经皮水分损失（TEWL）来确定。与对照组相比，五种 JP-8 成分都显著（P<0.05）增加了 TEWL。我们假设暴露面积为 0.25 m2，暴露 8 小时，从而量化吸收的化学物质的量。我们的研究结果表明，在脂肪族中，十三烷的渗透性大于其他物质，而在芳香族中，萘的渗透性大于其他物质。吸收的化学物质的量表明，应该监测十三烷、萘及其甲基衍生物是否存在潜在的全身毒性。
