Laboratory of Radiation Biology, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, 52-1, Yada, Shizuoka-shi 422-8526, Japan.
Environ Toxicol Pharmacol. 2002 Aug;12(1):37-42. doi: 10.1016/s1382-6689(02)00014-5.
The influence of environmental benzo[a]pyrene (BaP) contamination under ultraviolet A (UVA) on normal human skin fibroblasts was examined. Treatment of human skin fibroblasts with UVA in the presence of BaP induced cytotoxicity in a UVA- and BaP-dose-dependent manner, involving oxidative DNA damage (formation of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dG)). Singlet oxygen quenchers significantly inhibited the formation of 8-oxo-dG, whereas hydroxyl radical and superoixide anion radical scavengers showed no effect. N-Acetyl-l-cysteine prevented the formation of 8-oxo-dG. These findings suggested the possibility of increased carcinogenesis in the skin via singlet oxygen produced by sunlight plus environmental BaP contamination and the efficiency of anti-oxidant agents for its prevention.
研究了在紫外线 A（UVA）存在下环境苯并[a]芘（BaP）污染对正常人类皮肤成纤维细胞的影响。在 UVA 和 BaP 存在的情况下，用 UVA 处理人类皮肤成纤维细胞会导致细胞毒性，这是一种与 UVA 和 BaP 剂量相关的方式，涉及氧化 DNA 损伤（形成 8-氧-7,8-二氢-2'-脱氧鸟苷（8-oxo-dG））。单线态氧清除剂显著抑制 8-oxo-dG 的形成，而羟基自由基和超氧阴离子自由基清除剂则没有影响。N-乙酰-L-半胱氨酸可防止 8-oxo-dG 的形成。这些发现表明，通过阳光和环境 BaP 污染产生的单线态氧增加皮肤癌变的可能性，以及抗氧化剂预防其癌变的效率。
