Department of Biotechnology and Microbiology, Karnataka University, Dharwad 580003, India.
Environ Toxicol Pharmacol. 2004 Jan;15(2-3):71-7. doi: 10.1016/j.etap.2003.10.007.
Carbofuran, a systemic N-methyl carbamate pesticide was administered orally with an effective dose of 1.3mg/kg per day to hemiovariectomized (HOVX) mice for 5, 10, and 15 days. Sham-operated and HOVX control mice were administered a similar quantity of olive oil. The vaginal smear and body weight of the mice were recorded daily and mice were sacrificed on day 16. The results of the present study indicate that there is a significant decrease in the duration of the estrus with a concomitant significant increase in the duration of the diestrus with carbofuran treatment for 10 days. In the HOVX mice treated with carbofuran for 15 days there was a significant decrease in the length of the estrous cycle and the duration of the estrus and metestrus with a concomitant significant increase in the diestrus. There was a significant increase in the ovarian weight in HOVX control mice when compared to that of the sham operated control mice. HOVX mice treated with carbofuran for 10 and 15 days showed a significant decrease in the relative ovarian weight with a concomitant inhibition of compensatory ovarian hypertrophy in HOVX mice. There was also a significant decrease in the ovarian growth rate in relation to the contralateral ovary of the same animal. There was a significant decrease in the number of small and total number of healthy follicles with a concomitant significant increase in the number of medium, large and total number of atretic follicles in HOVX mice treated with carbofuran for 10 days. There was a significant decrease in the number of small, medium, large, and total number of healthy follicles with a concomitant significant increase in the number of medium, large, and total number of atretic follicles in HOVX mice treated with carbofuran for 15 days. The administration of carbofuran in the present study showed that there is no significant change in the body and organs weight such as uterus, kidney, adrenals, liver, spleen, thymus and thyroid in all the carbofuran-treated mice. The above findings such as disruption in the estrous cycle, inhibition of compensatory ovarian hypertrophy, and follicular toxicity may be due to a direct effect on the ovary or through the hypothalamo-hypophysial-ovarian axis.
克百威，一种系统性 N-甲基氨基甲酸酯类农药，以 1.3mg/kg 有效剂量经口给予单侧卵巢切除术（HOVX）小鼠，连续 5、10 和 15 天。假手术和 HOVX 对照组小鼠给予等量橄榄油。每日记录阴道涂片和小鼠体重，第 16 天处死小鼠。本研究结果表明，克百威处理 10 天可显著缩短发情期持续时间，同时显著延长间情期。15 天的克百威处理可显著缩短发情周期、发情和动情期的持续时间，同时显著延长间情期。与假手术对照组相比，单侧卵巢切除术的 HOVX 对照组小鼠卵巢重量显著增加。10 和 15 天的克百威处理 HOVX 小鼠的相对卵巢重量显著降低，同时抑制了 HOVX 小鼠的代偿性卵巢肥大。与同一动物对侧卵巢相比，卵巢生长速度也显著降低。10 天的克百威处理 HOVX 小鼠中，小卵泡和总健康卵泡数量显著减少，同时中型、大型和总闭锁卵泡数量显著增加。15 天的克百威处理 HOVX 小鼠中，小卵泡、中卵泡、大卵泡和总健康卵泡数量显著减少，中型、大型和总闭锁卵泡数量显著增加。本研究中克百威的给药表明，在所有接受克百威处理的小鼠中，子宫、肾脏、肾上腺、肝脏、脾脏、胸腺和甲状腺等器官的体重没有显著变化。发情周期紊乱、代偿性卵巢肥大抑制和卵泡毒性等上述发现可能是由于对卵巢的直接作用或通过下丘脑-垂体-卵巢轴。
