Department of Cell Biology, Biomedical Research Center, University of Rostock, Schillingallee 69, 18057 Rostock, Germany.
J Ethnopharmacol. 2011 Sep 2;137(2):1003-10. doi: 10.1016/j.jep.2011.07.023. Epub 2011 Jul 18.
The medicinal plants Hunteria umbellata (HUL), Cola lepidota (CCL), Persea americana leaf (PAL), Root bark of Persea americana (RPA) and Plukenetia conophora (PCL) are used in Nigerian traditional medicine for the treatment of cancer and cancer related diseases.
To scientifically evaluate the cell proliferative and apoptotic effects of the plants extracts using breast and osteocarcinoma cell lines, and also to identify the possible components via LC-MS to have a kind of chemical fingerprint.
The antiproliferative and apoptotic effects of methanolic extracts (10 μg/ml) of the five medicinal plants were subjected to in vitro evaluation using four cancer cell lines (breast-MCF-7 and BT-20; Osteocarcinoma-MG-63 and Saos-2) measured by flow cytometry. Non-tumorigenic controls MCF-12A and primary isolated osteoblasts (POB) were chosen to eliminate negative influence on healthy tissue.
Of the five extracts RPA demonstrated a significant (P<0.05) anti-proliferative activity against estrogen receptor positive breast cancer cell lines (MCF-7). The proliferative phase was decreased by 18%, whereas, a significant increase in cell proliferation (about 27%) was observed for RPA at a concentration of 10 μg/ml. PCL, CCL, HUL and PAL did not show marked inhibition of the proliferation of cell line MCF-7.
These results give suggestive evidence that the plant extracts exhibit some correlation between the claimed ethnomedicinal uses and the cell proliferative activity. RPA extract includes chemical compounds with estrogen-like activity and validates its potential use as anticancer agent, particularly against breast carcinoma; provided important information potentially helpful in drug designing and discovery. Further studies will involve the isolation of anti tumour compounds in RPA by LC-MS and detailed mechanism of anticancer activities.
在尼日利亚传统医学中，Hunteria umbellata（HUL）、Cola lepidota（CCL）、Persea americana 叶（PAL）、Persea americana 根皮（RPA）和 Plukenetia conophora（PCL）等药用植物被用于治疗癌症和与癌症相关的疾病。
使用乳腺癌和骨肉瘤细胞系，科学评估这些植物提取物的细胞增殖和凋亡作用，并通过 LC-MS 鉴定可能的成分，以获得一种化学指纹图谱。
采用流式细胞术，用四种癌细胞系（乳腺癌-MCF-7 和 BT-20；骨肉瘤-MG-63 和 Saos-2）体外评价五种药用植物甲醇提取物（10μg/ml）的抗增殖和凋亡作用。选择非致瘤性对照 MCF-12A 和原代成骨细胞（POB），以消除对健康组织的负面影响。
五种提取物中，RPA 对雌激素受体阳性乳腺癌细胞系（MCF-7）表现出显著的（P<0.05）抗增殖活性。增殖期减少了 18%，而在 10μg/ml 浓度下，RPA 观察到细胞增殖显著增加（约 27%）。PCL、CCL、HUL 和 PAL 对 MCF-7 细胞系的增殖没有明显抑制作用。
这些结果表明，植物提取物在声称的民族医学用途和细胞增殖活性之间存在一定的相关性。RPA 提取物包含具有雌激素样活性的化学化合物，验证了其作为抗癌剂的潜在用途，特别是对乳腺癌；提供了潜在有助于药物设计和发现的重要信息。进一步的研究将涉及通过 LC-MS 分离 RPA 中的抗肿瘤化合物，并详细研究其抗癌活性的机制。
