Aydemir Esra, Odabaş Köse Elif, Yavuz Mustafa, Kilit A Cansu, Korkut Alaaddin, Özkaya Gül Serap, Sarikurkcu Cengiz, Celep Mehmet Engin, Göktürk R Süleyman
Department of Biology, Faculty of Science, Akdeniz University, Antalya TR-07058, Turkey.
Medical Laboratory Program, Vocational School of Health Services, Akdeniz University, Antalya TR-07058, Turkey.
Plants (Basel). 2024 Feb 27;13(5):658. doi: 10.3390/plants13050658.
Since is a genus with many important medicinal plant species, the present work aimed to investigate the phytochemical composition and some biological activities of . The methanolic fractions of four organs (stems, flowers, leaves, root and whole plant) were quantified and identified by Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry (LC-ESI-MS/MS) analysis. Hesperidin, hyperoside, -hydroxybenzoic acid, protocatechuic acid and -coumaric acid were identified as main compounds among the extracts. Among all cells, leaf methanol (Lm) extract had the highest cytotoxic effect on HeLa cells (IC = 0.069 μg/mL). Hesperidin, the most abundant compound in extract, was found to show a strong negative correlation with the cytotoxic effect observed in HeLa cells according to Pearson correlation test results and to have the best binding affinity to targeted proteins by docking studies. The antimicrobial activity results indicated that the most susceptible bacterium against all extracts was identified as with 9-11 mm inhibition zone and 8192 mg/mL MIC value. As a result of the research, it was suggested that could be considered as a promising source that contributes to the fight against cancer.
由于[属名]是一个包含许多重要药用植物物种的属，本研究旨在调查[属名]的植物化学成分和一些生物活性。通过液相色谱电喷雾电离串联质谱（LC-ESI-MS/MS）分析对四个器官（茎、花、叶、根和全株）的甲醇提取物进行了定量和鉴定。橙皮苷、金丝桃苷、对羟基苯甲酸、原儿茶酸和对香豆酸被鉴定为提取物中的主要化合物。在所有细胞中，叶甲醇（Lm）提取物对HeLa细胞具有最高的细胞毒性作用（IC = 0.069 μg/mL）。根据Pearson相关性测试结果，橙皮苷是[属名]提取物中含量最丰富的化合物，发现其与在HeLa细胞中观察到的细胞毒性作用呈强烈负相关，并且通过对接研究表明其与靶向蛋白具有最佳的结合亲和力。抗菌活性结果表明，对所有提取物最敏感的细菌被鉴定为[细菌名称]，其抑菌圈为9 - 11毫米，最低抑菌浓度（MIC）值为8192毫克/毫升。研究结果表明，[属名]可被视为对抗癌症斗争有贡献的有前景的资源。
