Institute of Basic Theory China Academy of Chinese Medical Sciences, 16 Dong Zhi Men Nei Nan Xiao Jie, Dong Cheng District, Beijing 100700, China.
J Ethnopharmacol. 2011 Sep 2;137(2):992-7. doi: 10.1016/j.jep.2011.07.019. Epub 2011 Jul 18.
Angelicae Sinensis is a well known and commonly used traditional Chinese herbal medicine with many therapeutic effects such as neuroprotection, the promotion of hematopoiesis and the treatment of tumors.
Ferulic acid (FA) is the main active component in Angelicae Sinensis. Previous research has demonstrated that Angelicae Sinensis is able to induce angiogenesis in vivo. This study investigated the effects of FA on the proliferation of a human umbilical vein endothelial cell line (ECV304) with regard to the modulation of endothelial cells, which is a key step of angiogenesis.
ECV304 cells were incubated with FA at different dosages (0.1 μg/mL, 1 μg/mL and 10 μg/mL). A series of assays was used to detect the effects of FA on: (i) cell proliferation; (ii) DNA synthesis; (iii) cell-cycle distribution; and (iv) mRNA expression of cyclin D1 and vascular endothelial growth factor (VEGF). These were assessed using the Cell Counting Kit-8 (CCK-8), bromodeoxyuridine-enzyme-linked immunosorbent assay (BrdU-ELISA), flow cytometry and reverse transcription-polymerase chain reaction (RT-PCR), respectively.
The results showed that FA at a range of concentrations from 0.1 μg/mL to 10 μg/mL could markedly improve cell proliferation and DNA synthesis in a dose-dependent manner. Flow cytometry showed a significant decrease in the percentage of cells in the G(0)/G(1) phase and a significant increase in the percentage of cells in the S phase. Furthermore, we found that FA enhanced cyclin D1 and VEGF mRNA expression in ECV304 cells.
FA was able to promote ECV304 cells proliferation in vitro. This effect might be observed through the modulation of cyclin D1 and VEGF.
当归是一种著名的、常用的传统中药，具有多种治疗作用，如神经保护、促进造血和治疗肿瘤。
阿魏酸（FA）是当归的主要活性成分。先前的研究表明，当归能够在体内诱导血管生成。本研究探讨了 FA 对人脐静脉内皮细胞系（ECV304）增殖的影响，这是血管生成的关键步骤。
将 ECV304 细胞用 FA 在不同剂量（0.1μg/ml、1μg/ml 和 10μg/ml）孵育。采用一系列试验检测 FA 对以下方面的影响：（i）细胞增殖；（ii）DNA 合成；（iii）细胞周期分布；和（iv）细胞周期蛋白 D1 和血管内皮生长因子（VEGF）的 mRNA 表达。分别采用细胞计数试剂盒-8（CCK-8）、溴脱氧尿苷酶-酶联免疫吸附试验（BrdU-ELISA）、流式细胞术和逆转录聚合酶链反应（RT-PCR）进行评估。
结果表明，FA 在 0.1μg/ml 至 10μg/ml 的浓度范围内能够显著改善细胞增殖和 DNA 合成，呈剂量依赖性。流式细胞术显示，G0/G1 期细胞的比例显著降低，S 期细胞的比例显著增加。此外，我们发现 FA 增强了 ECV304 细胞中环蛋白 D1 和 VEGF 的 mRNA 表达。
FA 能够促进 ECV304 细胞在体外的增殖。这种作用可能是通过调节细胞周期蛋白 D1 和 VEGF 来实现的。
