Department of Food and Nutrition, Hung Kuang University, Taichung 433, Taiwan, ROC.
Environ Toxicol Pharmacol. 2005 Jul;20(1):135-41. doi: 10.1016/j.etap.2004.11.007. Epub 2005 Jan 13.
The dominant lethal assay was utilized to assess the reproductive performance in male mice, possible genetic hazards, and persistent damage of aluminum (Al). Al chloride, AlCl(3), was administered subcutaneously to CD-1 adult male mice at dosages of 0, 7, or 13mg Al/kg body weight/day for 2 weeks of pre-mating periods. Females were not dosed at any time during this study. At the end of the exposure period, each male was caged with three virgin females each day. The mean mating frequencies of the Al-treated groups reduced consistently from week 4 to 6, and a dramatic reduction in male fertility was also observed. However, the mating frequency restored to near normal control levels as the experiment terminated. Results showed significantly higher numbers of post-implantation losses, foetal mortality, and induced petechial haemorrhage; also significant decreases in body weights of viable foetus throughout weeks 3-8 in the Al-treated groups. The weights of the reproductive organs of the Al-dosed animals decreased significantly as Al accumulation increased in the testes. The spermatogenetic impairment within the seminiferous tubules was also apparent. Nevertheless, these disturbances disappeared at the end of the experiments. In summary, the results demonstrated that Al exerted substantial hazards on male reproductive function and produced genetic toxicity. However, these effects were found to be reversible.
利用显性致死试验评估了雄性小鼠的生殖性能、可能的遗传危害和铝（Al）的持续损伤。将氯化铝（AlCl(3)）以 0、7 或 13mg Al/kg 体重/天的剂量皮下注射给 CD-1 成年雄性小鼠，用于交配前 2 周。在这项研究中，女性在任何时候都没有接受剂量。在暴露期结束时，每天将每只雄性与三只处女雌性关在一个笼子里。Al 处理组的平均交配频率从第 4 周到第 6 周持续下降，并且观察到雄性生育力显著降低。然而，随着实验结束，交配频率恢复到接近正常对照组水平。结果表明，植入后损失、胎儿死亡率和诱导的点状出血的数量明显增加；在 Al 处理组中，第 3 周到第 8 周期间活胎的体重也明显下降。随着睾丸中 Al 积累的增加，Al 处理动物的生殖器官重量显著下降。在曲细精管内的精子发生损伤也很明显。然而，这些干扰在实验结束时消失了。总之，结果表明 Al 对雄性生殖功能有很大的危害，并产生遗传毒性。然而，这些影响被发现是可逆的。
