Botany Department, Faculty of Science, Tanta University, Tanta, Egypt.
Environ Toxicol Pharmacol. 2006 Jan;21(1):42-50. doi: 10.1016/j.etap.2005.06.006. Epub 2005 Aug 25.
In this investigation, the cyanobacterium Nostoc muscorum exhibited antagonistic activity against Gram-positive and Gram-negative bacteria and filamentous fungi. The results indicated that the active substance produced maximally after 12 days of incubation in shaken culture at 35°C, at pH 8.0 in BG-11 medium. The increase in nitrate concentration of the medium led to an increase in the antimicrobial production. Chloroform was the best solvent for extracting the active material. The antagonistic material was purified using thin layer chromatography. The compound showed maximum absorption at 240nm. Infrared (IR) and nuclear magnetic resonance (NMR) indicated presence of γOH, γCH aromatic, γCH aliphatic, γCN, γCO, γCC and CO. Mass spectroscopy indicated that its molecular weight is 279. The results also indicated that the compound is phenolic compound.
在这项研究中，蓝藻念珠藻对革兰氏阳性和革兰氏阴性细菌以及丝状真菌表现出拮抗活性。结果表明，在 BG-11 培养基中于 35°C 下摇床培养 12 天后，产生的活性物质最多，在 pH8.0 时达到最大值。培养基中硝酸盐浓度的增加导致抗菌产物的增加。氯仿是提取活性物质的最佳溶剂。采用薄层层析法对拮抗物质进行了纯化。该化合物在 240nm 处显示最大吸收。红外（IR）和核磁共振（NMR）表明存在γOH、γCH 芳族、γCH 脂肪族、γCN、γCO、γCC 和 CO。质谱表明其分子量为 279。结果还表明，该化合物是酚类化合物。
