Badji B, Zitouni A, Mathieu F, Lebrihi A, Sabaou N
Laboratoire de Recherche sur les Produits Bioactifs et la Valorisation de la Biomasse, Ecole Normale Supérieure de Kouba, Biskra.
Can J Microbiol. 2006 Apr;52(4):373-82. doi: 10.1139/w05-132.
During a search for nonpolyenic antifungal antibiotics, an actinomycete designated AC104 was isolated from a Saharan soil sample by a dilution agar plating method using a chitin - vitamins B medium supplemented with rifampicin. Isolate AC104 presented the morphological and the chemical characteristics of the genus Actinomadura. On the basis of 76 physiological tests and 16S rDNA analysis, this isolate was determined to be quite different from the known species of Actinomadura. It is active against filamentous fungi and both Gram-positive and Gram-negative bacteria. The production of antibiotic substances was investigated using several culture media. The highest antimicrobial activities were obtained on ISP2 medium. The benzenic extract contained five bioactive spots detected on thin layer chromatography plates. Among these antibiotics, a complex called 104A, which showed the more interesting antifungal activity, was selected and purified by reverse-phase high-pressure liquid chromatography. This complex is composed of four compounds. Ultraviolet-visible, infrared, mass, and 1H nuclear magnetic resonance spectroscopy studies showed that these molecules contain an aromatic ring substituted by aliphatic chains. These compounds differ from the known antibiotics produced by Actinomadura species.
在寻找非多烯类抗真菌抗生素的过程中，通过稀释琼脂平板法，使用添加了利福平的几丁质 - 维生素B培养基，从撒哈拉沙漠土壤样本中分离出了一株编号为AC104的放线菌。分离株AC104呈现出马杜拉放线菌属的形态和化学特征。基于76项生理测试和16S rDNA分析，确定该分离株与马杜拉放线菌属的已知物种有很大不同。它对丝状真菌以及革兰氏阳性和革兰氏阴性细菌均有活性。使用几种培养基对抗生素物质的产生进行了研究。在ISP2培养基上获得了最高的抗菌活性。苯提取物在薄层色谱板上检测到五个生物活性斑点。在这些抗生素中，选择了一种名为104A的复合物，它表现出更有趣的抗真菌活性，并通过反相高压液相色谱法进行了纯化。该复合物由四种化合物组成。紫外可见光谱、红外光谱、质谱和1H核磁共振光谱研究表明，这些分子含有被脂肪链取代的芳香环。这些化合物与马杜拉放线菌属物种产生的已知抗生素不同。
