Firat University, Faculty of Science, Biology Department, 23169-Elazıg, Turkey.
Environ Toxicol Pharmacol. 2007 Sep;24(2):79-85. doi: 10.1016/j.etap.2007.05.007. Epub 2007 May 24.
Resveratrol is a natural polyphenolic compound and it is found in number of edible plants, especially grapes and peanuts, has been shown to have anti-inflammatory, anti-oxidant, anti-tumor and anti-platelet activities. The aim of the study was to examine the effects of resveratrol on the level of malondialdehyde (MDA), homocysteine, cholesterol, GSH, GSSG and lipophylic vitamins in serum and erythrocytes of old female Wistar rats induced by the kidney carcinogen potassium bromate (KBrO(3)). In the study, total 30-old female Wistar rats were used, and the rats were randomly divided into three groups. The first group was used as a control, the second group KBrO(3) group, and third group R+KBrO(3). Rats in KBrO(3) and R+KBrO(3) groups were injected intraperitoneally a single dose KBrO(3) (80mg/kg) in physiologic saline buffer. After 2days, those in R+KBrO(3) group were intraperitoneally injected with resveratrol (33mg/kg) four times per week, and physiological saline was injected to control group rats. All the analysis was performed fully automatic with high performance liquid chromatography equipment. The results indicate that serum cholesterol level in the R+KBrO(3) group was higher than the control group (p<0.05), and level of the cholesterol in erythrocytes membranes was lower in the same group (p<0.01). The MDA level in serum and erythrocytes of the R+KBrO(3) were lower than the control and KBrO(3) groups (p<0.01). However, the MDA level in erythrocytes of the KBrO(3) group was high compared to the control group (p<0.05). Homocysteine and δ-tocopherol levels in serum of the R+KBrO(3) group were lower than the control group (p<0.05, p<0.001). α-Tocopherol level in serum and erythrocytes of the KBrO(3) group was lower than the control group (p<0.05), whereas its level was not found to differ between the control and R+KBrO(3) groups. GSH and GSSG levels in the KBrO(3) group of erythrocytes were higher than control group (p<0.05, p<0.01), however the ratio GSH/GSSG in the same group was lower than control group. In conclusion, our results confirm that the lipid peroxidation formation in serum and erythrocytes of old female Wistar rats by induced the KBrO(3) is prevented by the resveratrol. It was observed that the antioxidant capacity of erythrocytes was protected by resveratrol.
白藜芦醇是一种天然多酚化合物，存在于许多可食用植物中，尤其是葡萄和花生，已被证明具有抗炎、抗氧化、抗肿瘤和抗血小板作用。本研究的目的是研究白藜芦醇对肾脏致癌剂溴酸钾（KBrO3）诱导的老年雌性 Wistar 大鼠血清和红细胞中丙二醛（MDA）、同型半胱氨酸、胆固醇、GSH、GSSG 和脂溶性维生素水平的影响。在这项研究中，使用了 30 只老年雌性 Wistar 大鼠，将大鼠随机分为三组。第一组为对照组，第二组为 KBrO3 组，第三组为 R+KBrO3 组。KBrO3 和 R+KBrO3 组的大鼠腹腔内注射单剂量 KBrO3（80mg/kg）生理盐水缓冲液。2 天后，R+KBrO3 组大鼠每周腹腔内注射白藜芦醇（33mg/kg）4 次，对照组大鼠注射生理盐水。所有分析均使用高效液相色谱设备全自动进行。结果表明，R+KBrO3 组大鼠血清胆固醇水平高于对照组（p<0.05），同一组大鼠红细胞膜上的胆固醇水平较低（p<0.01）。R+KBrO3 组大鼠血清和红细胞中的 MDA 水平低于对照组和 KBrO3 组（p<0.01）。然而，KBrO3 组大鼠红细胞中的 MDA 水平高于对照组（p<0.05）。R+KBrO3 组大鼠血清同型半胱氨酸和δ-生育酚水平低于对照组（p<0.05，p<0.001）。KBrO3 组大鼠血清和红细胞中的α-生育酚水平低于对照组（p<0.05），但对照组和 R+KBrO3 组之间的水平无差异。KBrO3 组大鼠红细胞中的 GSH 和 GSSG 水平高于对照组（p<0.05，p<0.01），但同一组的 GSH/GSSG 比值低于对照组。综上所述，我们的研究结果证实，白藜芦醇可预防 KBrO3 诱导的老年雌性 Wistar 大鼠血清和红细胞中的脂质过氧化形成。观察到白藜芦醇可保护红细胞的抗氧化能力。
