Program of Post-Graduation in Pharmaceutical Sciences, Federal University of Santa Maria, Campus Camobi, Santa Maria, RS 97105-900, Brazil.
Molecules. 2012 May 14;17(5):5757-69. doi: 10.3390/molecules17055757.
Flavonoids are claimed to protect against cardiovascular disease, certain forms of cancer and ageing, possibly by preventing initial DNA damage. Therefore, we investigated the protective effects of crude extract, ethyl acetate fraction and flavonoids (quercetin, quercitrin, isoquercitrin and rutin) isolated from the leaves from Scutia buxifolia against chromosome damage induced by H₂O₂ in human lymphocytes by analyzing cellular growth rate, cell viability, mitotic index and chromosomal instability. We found a differential response among the compounds tested, with the ethyl acetate fraction being more effective than the crude extract, a difference perhaps related to the presence of the antioxidants identified and quantified by HPLC/DAD. In general, quercetin, isoquercitrin and rutin recovered the mitotic index and chromosomal instability more than quercitrin after treatment with hydrogen peroxide.
类黄酮据称可以预防心血管疾病、某些形式的癌症和衰老，其作用机制可能是防止初始 DNA 损伤。因此，我们通过分析细胞生长率、细胞活力、有丝分裂指数和染色体不稳定性，研究了从 Scutia buxifolia 叶中分离得到的粗提物、乙酸乙酯部分和类黄酮（槲皮素、槲皮苷、异槲皮苷和芦丁）对 H₂O₂诱导的人淋巴细胞染色体损伤的保护作用。我们发现，在所测试的化合物中存在差异反应，乙酸乙酯部分比粗提取物更有效，这种差异可能与通过 HPLC/DAD 鉴定和定量的抗氧化剂的存在有关。一般来说，与槲皮苷相比，在用过氧化氢处理后，槲皮素、异槲皮苷和芦丁更能恢复有丝分裂指数和染色体不稳定性。
