Karbownik Małgorzata, Stasiak Magdalena, Zygmunt Arkadiusz, Zasada Krzysztof, Lewiński Andrzej
Department of Endocrinology and Metabolic Diseases, Medical University of Łódź, Polish Mother's Memorial Hospital--Research Institute, 281/289 Rzgowska Street, 93-338 Łódź, Poland.
Cell Biochem Funct. 2006 Nov-Dec;24(6):483-9. doi: 10.1002/cbf.1321.
Potassium bromate (KBrO(3)) is classified as a carcinogenic agent. KBrO(3) induces tumors and pro-oxidative effects in kidneys. Melatonin is a well known antioxidant and free radical scavenger. Indole-3-propionic acid (IPA), an indole substance, also reveals antioxidative properties. Recently, some antioxidative effects of propylthiouracil (PTU)-an antithyroid drug-have been found. The aim of the study was to compare protective effects of melatonin, IPA, and PTU against lipid peroxidation in the kidneys and blood serum and, additionally, in the livers and the lungs, collected from rats, pretreated with KBrO(3). Male Wistar rats were administered KBrO(3) (110 mg/kg b.w., i.p., on the 10th day of the experiment) and/or melatonin, or IPA (0.0645 mmol/kg b.w., i.p., twice daily, for 10 days), or PTU (0.025% solution in drinking water, for 10 days). The level of lipid peroxidation products-malondialdehyde + 4-hydroxyalkenals (MDA + 4-HDA)-was measured spectrophotometrically in thyroid homogenates. KBrO(3), when injected to rats, significantly increased lipid peroxidation in the kidney homogenates and blood serum, but not in the liver and the lung homogenates. Co-treatment with either melatonin or with IPA, but not with PTU, decreased KBrO(3)-induced oxidative damage to lipids in the rat kidneys and serum. In conclusion, melatonin and IPA, which prevent KBrO(3)-induced lipid peroxidation in rat kidneys, may be of great value as protective agents under conditions of exposure to KBrO(3).
溴酸钾（KBrO₃）被归类为致癌物质。溴酸钾可诱发肾脏肿瘤并产生促氧化作用。褪黑素是一种知名的抗氧化剂和自由基清除剂。吲哚 - 3 - 丙酸（IPA），一种吲哚类物质，也具有抗氧化特性。最近，已发现抗甲状腺药物丙硫氧嘧啶（PTU）具有一些抗氧化作用。本研究的目的是比较褪黑素、IPA和PTU对从用溴酸钾预处理的大鼠收集的肾脏、血清、肝脏和肺中脂质过氧化的保护作用。雄性Wistar大鼠在实验第10天腹腔注射溴酸钾（110 mg/kg体重）和/或褪黑素，或IPA（0.0645 mmol/kg体重，腹腔注射，每日两次，共10天），或PTU（饮用水中0.025%的溶液，共10天）。采用分光光度法测定甲状腺匀浆中脂质过氧化产物丙二醛 + 4 - 羟基烯醛（MDA + 4 - HDA）的水平。给大鼠注射溴酸钾后，肾脏匀浆和血清中的脂质过氧化显著增加，但肝脏和肺匀浆中未增加。与褪黑素或IPA联合处理可降低溴酸钾诱导的大鼠肾脏和血清中脂质的氧化损伤，但与PTU联合处理则无此效果。总之，褪黑素和IPA可预防溴酸钾诱导的大鼠肾脏脂质过氧化，在接触溴酸钾的情况下作为保护剂可能具有重要价值。
