Sector of Disaster Medicine, Medical University-Pleven, 5800 Pleven, Bulgaria.
Environ Toxicol Pharmacol. 2007 Sep;24(2):167-73. doi: 10.1016/j.etap.2007.04.010. Epub 2007 May 18.
Paraquat is a very toxic herbicide and a dangerous pollutant of the environment. It forms reactive oxygen species and increases the lipid peroxidation in the pulmonary cells. Our aim in this study was to estimate the protective effects of the lazaroid U-74389G possessing antilipidperoxidation activity and membrane-stabilizing effect. The experiment was carried out with 96 male Wistar rats. Paraquat dichloride was administered orally at 80mg/kg. The lazaroid U-74389G was injected intraperitoneally twice - 2h before receiving the paraquat with 10mg/kg and four hours later with 5mg/kg. Isolated application of paraquat increased enzyme activities of lactate dehydrogenase (LDH) and acid phosphatase (AcP) and the total protein content in bronchoalveolar lavage fluid (BALF). In the same experimental group the number of polymorphonuclear cells (PMNs) in BALF is elevated significantly on days 1 and 3. The combined treatment with paraquat and U-74389G did not increase the total protein content and the number of PMNs and it elevated the enzyme activities of LDH and AcP significantly less than the alone application of paraquat. It is concluded that the lazaroid U-74389G reduces the pneumotoxic effects of paraquat, estimated by sensitive cytologic and biochemical markers in BALF. The protective effect of U-74389G is well-expressed until day 3 after the treatment.
百草枯是一种毒性很强的除草剂，也是一种危险的环境污染物。它会形成活性氧物质，增加肺细胞中的脂质过氧化。我们在这项研究中的目的是评估具有抗脂质过氧化和膜稳定作用的 Lazaroid U-74389G 的保护作用。该实验使用了 96 只雄性 Wistar 大鼠。口服给予百草枯二氯盐 80mg/kg。Lazaroid U-74389G 两次腹腔注射，分别在接受百草枯前 2 小时给予 10mg/kg，4 小时后给予 5mg/kg。单独应用百草枯会增加乳酸脱氢酶（LDH）和酸性磷酸酶（AcP）的酶活性以及支气管肺泡灌洗液（BALF）中的总蛋白含量。在同一实验组中，BALF 中的多形核细胞（PMN）数量在第 1 天和第 3 天显著升高。与单独应用百草枯相比，百草枯和 U-74389G 的联合治疗并没有增加总蛋白含量和 PMN 数量，并且明显降低了 LDH 和 AcP 的酶活性。结论是 Lazaroid U-74389G 通过 BALF 中的敏感细胞和生化标志物降低了百草枯的肺毒性作用。U-74389G 的保护作用在治疗后第 3 天仍表现良好。
