慢性给予阿拉伯茶粗提物后对大鼠的遗传毒性作用。
Genotoxic effect of Catha edulis (khat) crude extract after sub-chronic administration in rats.
机构信息
Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia; Department of Clinical Biochemistry, Faculty of Medicine and Health Sciences, University of Sana'a, Sana'a, Yemen.
出版信息
Environ Toxicol Pharmacol. 2008 May;25(3):298-303. doi: 10.1016/j.etap.2007.10.032. Epub 2007 Oct 26.
The aim of this study was to evaluate the genotoxic effects of a crude extract of khat (Catha edulis, Forsk) leaves in rats. Two groups were fed khat crude extract, 1000 and 2000mg/kg body weight, for 90 days and were compared with a control group. The alkaline (pH>13) version of comet assay was used in this study. However, no previous published work has been undertaken and showed the effect of khat on DNA migration in the comet assay. To compare the comet assay results with another genetic endpoint, blood samples were analyzed for chromosomal aberrations. These results showed no DNA damage detected using comet assay in both the khat treated groups, while the results of chromosomal aberrations assay showed a significant increase (P<0.05) in the 2000mg/kg body weight treated group compared to the control group.
本研究旨在评估阿拉伯茶（Catha edulis，Forsk）叶粗提物对大鼠的遗传毒性作用。两组大鼠分别以 1000 和 2000mg/kg 体重灌胃阿拉伯茶粗提物 90 天，并与对照组进行比较。本研究采用碱性（pH＞13）彗星试验。然而，之前没有发表过关于阿拉伯茶对彗星试验中 DNA 迁移影响的研究。为了将彗星试验结果与另一个遗传终点进行比较，对血液样本进行了染色体畸变分析。彗星试验结果显示，两个阿拉伯茶处理组均未检测到 DNA 损伤，而染色体畸变试验结果显示，2000mg/kg 体重处理组与对照组相比，染色体畸变显著增加（P＜0.05）。
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