Dip. di Biologia ed Evoluzione, Sez. Risorse Agrotecnologiche e Farmaceutiche AgriUnife, Università degli Studi di Ferrara, Italy.
Environ Toxicol Pharmacol. 2009 Jan;27(1):39-48. doi: 10.1016/j.etap.2008.08.002. Epub 2008 Aug 19.
Essential oils from aerial parts of Piper aduncum (Matico) and Piper obliquum (Anis del Oriente) of ecuadorian origin were analyzed by GC-FID, GC-MS, (13)C NMR and their biological and pharmacological activities were assessed. Chemical composition proved to be unusually different from previous reports for safrole-rich P. obliquum (45.8%), while P. aduncum main constituent was dillapiol (45.9%). No genotoxic activity was found in the Ames/Salmonella typhimurium (TA98 and TA100) assay, either with or without S9 activation. Mutagen-protective properties, evaluated using sodium azide, 2-nitrofluorene and 2-aminoanthracene as mutagens/promutagens, was observed against promutagen 2-aminoanthracene, likely in consequence of microsomial deactivation. Antimicrobial assays have been performed on Gram+/Gram- bacteria, dermatophyte and phytopathogenic fungi and best results were provided by P. aduncum against fungal strains with complete inhibition at 500μg/ml. Preliminary analgesic and antithrombotic activities evidenced the absence of the former in hot plate and edema assays and a limited antiplatelet action against three different agonists (ADP, AA and U46619). Both oils have a very limited antioxidant capacity.
产自厄瓜多尔的 Piper aduncum（Matico）和 Piper obliquum（Anis del Oriente）的地上部分精油通过 GC-FID、GC-MS、(13)C NMR 进行了分析，并评估了它们的生物和药理学活性。化学成分与之前富含 safrole 的 P. obliquum（45.8%）的报告异常不同，而 P. aduncum 的主要成分是 dillapiol（45.9%）。在用 Ames/Salmonella typhimurium（TA98 和 TA100）检测试剂盒进行的试验中，无论是有还是没有 S9 激活，都没有发现遗传毒性。在用叠氮化钠、2-硝基芴和 2-氨基蒽作为诱变剂/促变剂评估的诱变保护特性中，观察到对促变剂 2-氨基蒽具有保护作用，可能是由于微粒体失活。对革兰氏阳性/革兰氏阴性细菌、皮肤真菌和植物病原菌真菌进行了抗菌试验，P. aduncum 对真菌菌株的抑制效果最好，在 500μg/ml 时完全抑制。初步的镇痛和抗血栓形成活性表明，在热板和水肿试验中前者不存在，并且对三种不同激动剂（ADP、AA 和 U46619）的抗血小板作用有限。两种油的抗氧化能力都非常有限。
