Master Program of Natural Pharmaceutical Products, Universidad Politécnica Salesiana, Avenida 12 de Octubre N2422 y Wilson, Quito 170109, Ecuador.
Group of Research and Development in Science Applied to Biological Resourses, Universidad Politécnica Salesiana, Avenida 12 de Octubre N2422 y Wilson, Quito 170109, Ecuador.
Molecules. 2023 Feb 11;28(4):1741. doi: 10.3390/molecules28041741.
The Amazonian region of Ecuador has an extremely rich vegetal biodiversity, and its inhabitants have proven to have a millennial ancestral knowledge of the therapeutic and medicinal use of these resources. This work aimed to evaluate the chemical composition and biological activity of the essential oil obtained from the medicinal plant (Sw.) Kuntze, which is widely spread in tropical and subtropical America. This species is traditionally used for treating respiratory and digestive diseases and is also known for its analgesic properties. Most of the molecules detected on a non-polar column were ethyl cinnamate 21.4%, pulegone 20.76%, methyl cinnamate 16.68%, caryophyllene 8.17%, β-selinene 7.92% and menthone 7.51%, while those detected on a polar column were: pulegone 29.90%, ethyl cinnamate 18.75%, methyl cinnamate 13.82%, caryophyllene 10.0% and menthone 8.04%. The antioxidant activity by the assays, DPPH (2.2-diphenyl-1-picrylhydrazyl) and ABTS (2.2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)), shows the following values of 50% inhibition of oxidation, IC50 DPPH 1.77 mg/mL, IC50 ABTS 0.06 mg/mL, which, compared to the essential oil of (natural positive control), turn out to be less active. Bioautography indicates that the molecules responsible for the antioxidant activity are derived from cinnamic acid: ethyl cinnamate and methyl cinnamate, and caryophyllene. The antimicrobial activity on the nine microorganisms evaluated shows bacterial growth inhibitory concentrations ranging from 13.6 mg/mL for ATCC 14990 to 3.1 mg/mL for ATCC 10231; the results are lower than those of the positive control. Bioautography assigns antimicrobial activity to caryophyllene. The results indicate a very interesting activity of the essential oil and several of its molecules, validating the traditional use and the importance of this medicinal plant from Ecuador.
厄瓜多尔亚马逊地区拥有极其丰富的植物生物多样性，其居民拥有千年的传统医学知识，了解这些资源的治疗和药用价值。这项工作旨在评估从药用植物 （Sw.）Kuntze 中获得的精油的化学成分和生物活性，该植物广泛分布在热带和亚热带美洲。该物种传统上用于治疗呼吸道和消化系统疾病，也因其止痛特性而闻名。在非极性柱上检测到的大多数分子是乙基肉桂酸 21.4%、侧柏酮 20.76%、甲基肉桂酸 16.68%、丁香烯 8.17%、β-榄烯 7.92%和薄荷酮 7.51%，而在极性柱上检测到的是：侧柏酮 29.90%、乙基肉桂酸 18.75%、甲基肉桂酸 13.82%、丁香烯 10.0%和薄荷酮 8.04%。抗氧化活性测定结果表明，DPPH（2.2-二苯基-1-苦基肼）和 ABTS（2.2'-氮杂-双（3-乙基苯并噻唑啉-6-磺酸））的 50%氧化抑制率分别为 IC50 DPPH 1.77 mg/mL 和 IC50 ABTS 0.06 mg/mL，与 （天然阳性对照）的精油相比，活性较低。生物自显影表明，抗氧化活性的分子源自肉桂酸：乙基肉桂酸和甲基肉桂酸，以及丁香烯。对评价的九种微生物的抗菌活性表明，细菌生长抑制浓度范围为 13.6 mg/mL 用于 ATCC 14990 至 3.1 mg/mL 用于 ATCC 10231；结果低于阳性对照。生物自显影将抗菌活性分配给丁香烯。结果表明，精油及其几种分子具有非常有趣的活性，验证了厄瓜多尔这种药用植物的传统用途和重要性。
