Unité de Pharmacognosie/Biologie Moléculaire (99/UR/07-03), Faculté de Pharmacie de Monastir, Rue Avicenne, 5000 Monastir, Tunisia.
Environ Toxicol Pharmacol. 2009 Mar;27(2):187-94. doi: 10.1016/j.etap.2008.10.010. Epub 2008 Nov 6.
The SOS-chromotest in Escherichia coli is a widely used bacterial genotoxicity assay to test potential carcinogens. The aim of this work is to evaluate the genotoxic and antigenotoxic activities of essential oils obtained from aerial parts of Pituranthos chloranthus. The tested essential oils were not genotoxic towards both E. coli PQ37 and PQ35 strains. These essential oils reduced significantly Nifuroxazide and H(2)O(2)-induced genotoxicity. Essential oils showed a protective effect against damages induced by radicals, obtained from the photolysis of H(2)O(2), on DNA plasmid through free radical scavenging mechanisms. The scavenging capacity of these essential oils was also estimated by evaluating the inhibition of ABTS(+.) radical.
SOS 显色试验是一种广泛应用于细菌遗传毒性检测的方法，用于测试潜在的致癌物质。本研究旨在评估从苦玄参地上部分提取的精油的遗传毒性和抗原毒性活性。测试的精油对大肠杆菌 PQ37 和 PQ35 菌株均无遗传毒性。这些精油能显著降低硝呋太尔和 H₂O₂诱导的遗传毒性。精油通过自由基清除机制，对由 H₂O₂光解产生的自由基引起的 DNA 质粒损伤具有保护作用。通过评估对 ABTS(+)自由基的抑制作用，来估算这些精油的清除能力。
