Institute of Urban and Environment Science, Central China normal University, Wuhan 430079, PR China; School of Life Science, Hubei University, Wuhan 430062, PR China.
Environ Toxicol Pharmacol. 2009 Mar;27(2):283-6. doi: 10.1016/j.etap.2008.11.003. Epub 2008 Nov 21.
The comparative molluscicidal activities of Arisaema erubescens tuber extracts and Nerium indicum leaf extracts mixed with Streptomycete violacerruber dilution (SD) against the snail Oncomlania hupensis and the responses of the isozymes, esterase (EST) and superoxide dismutase (SOD) to the A. erubescens extracts and the mixtures were investigated. The molluscicidal activity of A. erubescens water extracts mixed with S. violacerruber dilution was 4-5 times higher than a single A. erubescens or S. violacerruber dilution after 24-h exposure, and is also higher than that of N. indicum leaf water extracts mixed with S. violacerruber dilution. At the end of exposure to the N-butanol extracts of A. erubescens tubers (NEAT), the EST activity in snail liver decreased and some enzyme bands (EST 1 and EST 3 in exposure to NEAT) disappeared but the activities of SOD 1 increased. The effect was more obvious in mixture treatment than in single NEAT or SD treatment. The results indicated that molluscicidal activities of plant and microorganism could be more effective than single plant. The decline of the detoxic ability in snail liver cells could be the reason of the snail dying.
研究了藜芦 Arisaema erubescens 块茎提取物和夹竹桃 Nerium indicum 叶提取物与 Streptomyces violacerruber 稀释液（SD）混合对福寿螺 Oncomlania hupensis 的比较杀螺活性，以及对同工酶酯酶（EST）和超氧化物歧化酶（SOD）的反应。藜芦水提取物与 S. violacerruber 稀释液混合后的杀螺活性在 24 小时暴露后比单一藜芦或 S. violacerruber 稀释液高 4-5 倍，也高于夹竹桃叶水提取物与 S. violacerruber 稀释液混合后的杀螺活性。在暴露于藜芦块茎正丁醇提取物（NEAT）结束时，蜗牛肝脏中的 EST 活性下降，一些酶带（暴露于 NEAT 时的 EST 1 和 EST 3）消失，但 SOD 1 的活性增加。与单一 NEAT 或 SD 处理相比，混合物处理的效果更为明显。结果表明，植物和微生物的杀螺活性可能比单一植物更有效。蜗牛肝细胞解毒能力的下降可能是蜗牛死亡的原因。
