Wang Hong, Cai Wei-Min, Wang Wan-Xian, Yang Jian-Min
School of Environmental Science and Engineering, Shanghai Jiaotong University, Shanghai 200240, China.
Biomed Environ Sci. 2006 Aug;19(4):245-8.
To evaluate the molluscicidal activities of three Chinese plants N. indicum Mill, R stenoptera DC, and R. japonicum Houtt, and to clarify the molluscicidal mechanism.
N-butanol extracts and water extracts of the three plants were obtained. The reactions of EST isozyme, glycogen and total protein of snails to the plant extracts were studied.
EST electrophoresis showed that EST was an important antidotal enzyme system and reacted strongly to environment. EST changed greatly during the whole exposure period so that it could be viewed as a pathological index of toxicity. Extracts decreased the glycogen content of the snails' soft tissues greatly, and also the protein content.
All extracts show strong molluscicidal activity. The LD50 value of the water extract of N. indicum Mill is as low as 13.2 mg/L. EST can be viewed as a pathological index of toxicity. The energy metabolism abnormity is the key reason for the molluscicidal activities. The biochemical mechanism needs further research.
评价三种中国植物（印度楝、窄叶鼠李和日本鼠李）的杀螺活性，并阐明其杀螺机制。
获得这三种植物的正丁醇提取物和水提取物。研究了蜗牛的酯酶同工酶、糖原和总蛋白对植物提取物的反应。
酯酶电泳显示酯酶是一种重要的解毒酶系统，对环境反应强烈。在整个暴露期间酯酶变化很大，因此可将其视为毒性的病理指标。提取物极大地降低了蜗牛软组织中的糖原含量，也降低了蛋白质含量。
所有提取物均表现出较强的杀螺活性。印度楝水提取物的半数致死剂量（LD50）低至13.2毫克/升。酯酶可被视为毒性的病理指标。能量代谢异常是杀螺活性的关键原因。其生化机制需要进一步研究。
