Department of Clinical Neurosciences, Neurology Unit, Addenbrookes Hospital, University of Cambridge, Cambridge, UK; Angiogenesis and Toxicology Research Laboratory, Department of Pharmacology and Toxicology, University of Veterinary and Animal Sciences, Lahore, Pakistan; Biosafety Research Institute and College of Veterinary Medicine, Chonbuk National University, Jeonju, 561-756, South Korea.
Environ Toxicol Pharmacol. 2009 May;27(3):373-83. doi: 10.1016/j.etap.2008.12.003. Epub 2008 Dec 25.
Vehicle exhaust from traffic is a widespread air pollutant. The use of 3-wheel auto-rickshaws powered by a 2-stroke engine is widespread in south Asia; exhaust from these vehicles may cause different types of toxicities resulting in different pathologies. The aim of this study was to explore the association between exposure to 2-stroke auto-rickshaw smoke solution (2SARSS) and wound healing. The in vivo model of wound healing was customized to evaluate different stages of wound healing: dermal matrix regeneration, re-epithelialization, and neovascularization. A total of 72 adult mice were divided into 8 groups and exposed to 2SARSS for 12 days. A highly significant reduction (p<0.001) in wound closure was observed among all 2SARSS-treated groups at day 8 post-wounding. Histological examination revealed a significant delay in the outcome of re-epithelialization, dermal matrix regeneration, and maturation of collagen bundles among all 2SARSS-exposed wounds. Delayed activation of neovascularization was seen in the 2SARSS-treated groups at day 12 post-wounding. The Abbot curve, angular spectrum, and several other 3D surface parameters of reverse wound topographies revealed a highly significant reduction (p<0.001) in angiogenesis. These results demonstrate that application of 2SARSS causes a substantial delay in the progression of angiogenesis, resulting in delayed onset of wound healing. These observations validate the damaging effects of 2SARSS on wound healing. Thus, people who are directly or indirectly exposed to this toxic exhaust are expected to have delayed wound healing, which could result in chronic wounds.
交通车辆尾气是一种广泛存在的空气污染物。在南亚，使用由二冲程发动机驱动的三轮人力车非常普遍；这些车辆的尾气可能会导致不同类型的毒性，从而导致不同的病理变化。本研究旨在探索接触二冲程人力车尾气溶液（2SARSS）与伤口愈合之间的关系。我们定制了体内伤口愈合模型，以评估伤口愈合的不同阶段：真皮基质再生、再上皮化和新血管生成。总共将 72 只成年小鼠分为 8 组，并暴露于 2SARSS 中 12 天。在受伤后第 8 天，所有 2SARSS 处理组的伤口闭合率均显著降低（p<0.001）。组织学检查显示，所有暴露于 2SARSS 的伤口中，再上皮化、真皮基质再生和胶原束成熟的结果均明显延迟。在受伤后第 12 天，2SARSS 处理组中可见新血管生成的延迟激活。反向伤口形貌的 Abbott 曲线、角谱和其他几个 3D 表面参数显示，血管生成显著减少（p<0.001）。这些结果表明，应用 2SARSS 会导致血管生成的显著延迟，从而导致伤口愈合的延迟。这些观察结果验证了 2SARSS 对伤口愈合的有害影响。因此，直接或间接暴露于这种有毒废气的人预计会出现伤口愈合延迟，从而导致慢性伤口。
