Department of Clinical Neurosciences, R3 Stroke Unit, Addenbrookes Hospital, University of Cambridge, Cambridge, CB2 0QQ, UK; Angiogenesis and Toxicology Research Laboratory, Department of Pharmacology and Toxicology, University of Veterinary and Animal Sciences, Lahore, Pakistan; Biosafety Research Institute and College of Veterinary Medicine, Chonbuk National University, Jeonju, 561-756, South Korea.
Environ Toxicol Pharmacol. 2009 Nov;28(3):323-32. doi: 10.1016/j.etap.2009.05.005. Epub 2009 May 20.
Automobile exhaust constituents contribute significantly to air pollution in urban areas and compressed natural gas (CNG) is considered one of the most promising fuel alternatives for the future. CNG-powered four-stroke engine auto-rickshaws are ubiquitous in South Asian cities as taxi and for commercial transportation. Automotive exhaust contains several toxins, which are overwhelmingly toxic to the processes of wound healing. By utilizing the in vivo mouse model of wound healing, this report analyzes the effects of CNG-powered four-stroke auto-rickshaws smoke solution (4SARSS) on different events of wound healing; dermal matrix regeneration, re-epithelialization and neovascularization. A total of 72 adult mice, divided in eight groups were exposed to 4SARSS for 12 days. A highly significant reduction (P<0.001) in wound closure was observed among all 4SARSS treated groups, at each time point of the experiment. An immature development in both the neoepidermis and the neodermis was observed among all 4SARSS treated wounds with defective re-epithelialization, dermal matrix regeneration and maturation of collagen bundles. Abbott curve, angular spectrum, 3D surface topographies, and histological investigations of wounds explicated highly significant activation (P<0.001) of delayed-neovascularization among 4SARSS treated wounds. All these annotations advocate excessive toxicity of emission from CNG-powered auto-rickshaws to the process of wound healing and people occupationally exposed to this toxic emissions may suffer varying degree of delayed wound healing.
汽车尾气成分对城市空气污染有重大贡献，压缩天然气（CNG）被认为是未来最有前途的燃料替代品之一。CNG 动力四冲程发动机人力车在南亚城市作为出租车和商业运输工具无处不在。汽车尾气含有几种毒素，对伤口愈合过程有极强的毒性。本报告利用体内小鼠伤口愈合模型，分析了 CNG 动力四冲程人力车烟雾溶液（4SARSS）对伤口愈合不同事件的影响；真皮基质再生、再上皮化和新血管形成。总共 72 只成年小鼠分为 8 组，暴露于 4SARSS 中 12 天。在实验的每个时间点，所有接受 4SARSS 治疗的组中，伤口闭合的速度都显著降低（P<0.001）。所有接受 4SARSS 治疗的伤口中，neoepidermis 和 neodermis 的发育都不成熟，表现为再上皮化、真皮基质再生和胶原束成熟缺陷。Abbott 曲线、角谱、3D 表面形貌和伤口的组织学研究表明，4SARSS 治疗的伤口中存在明显的延迟性新血管形成激活（P<0.001）。所有这些注释都表明，CNG 动力人力车排放物对伤口愈合过程具有过度毒性，职业接触这种毒性排放物的人可能会遭受不同程度的延迟性伤口愈合。
