Department of Clinical Neurosciences, Neurology Unit, Addenbrookes Hospital, University of Cambridge, UK.
Biomed Environ Sci. 2009 Aug;22(4):278-87. doi: 10.1016/S0895-3988(09)60057-X.
Wound healing in the skin is a multifarious orchestration of cellular processes and cigarette smoking may be a cause for delayed wound healing. The aim of this study was to investigate the plausible association between exposures of cigarette total particulate matter (TPM) and wound healing.
An in vivo wound healing model of mice was established for determination of assorted events of wound healing, dermal matrix regeneration, re-epithelialization, and neovascularization. A total of 72 adult mice, separated in eight groups, were exposed to TPM for 12 days.
A highly considerable diminution in wound closure (P < 0.001) was pragmatic among all TPM-treated mice from day 6 to day 8 post-wounding. Histological investigations unveiled a noteworthy impede in the outcome of re-epithelialization, dermal matrix regeneration and maturation of collagen bundles among all TPM-exposed wounds. Delayed commencement of neovascularization was pragmatic among all TPM-treated mice, on day 12 post wounding. Abbot curve, angular spectrum, and other different parameters of 3D surface behavior of wounds revealed a very highly significant reduction (P < 0.001) in angiogenesis on days 6 and 8 post-wounding, which points that application of TPM instigates extensive delay in trigging the progression of angiogenesis, resulting in delayed onset of wound healing.
Our annotations validate the damaging effects of TPM on wound healing and excessive use of TPM may lead to the production of chronic wounds and oral ulcers.
皮肤的伤口愈合是一个复杂的细胞过程的协调，吸烟可能是导致伤口愈合延迟的一个原因。本研究的目的是探讨香烟总颗粒物（TPM）暴露与伤口愈合之间可能存在的关联。
建立了一种体内小鼠伤口愈合模型，以确定伤口愈合、真皮基质再生、再上皮化和新生血管形成的各种事件。将 72 只成年小鼠分为 8 组，分别暴露于 TPM 12 天。
所有 TPM 处理的小鼠从伤后第 6 天到第 8 天，伤口闭合率（P < 0.001）显著降低。组织学研究表明，所有 TPM 暴露的伤口中，再上皮化、真皮基质再生和胶原束成熟的结果显著受阻。所有 TPM 处理的小鼠在伤后第 12 天开始出现新生血管化延迟。伤后第 6 天和第 8 天，伤口的 3D 表面行为的 Abbott 曲线、角谱和其他不同参数显示，血管生成显著减少（P < 0.001），这表明 TPM 的应用引发了血管生成进展的广泛延迟，导致伤口愈合延迟。
我们的注释验证了 TPM 对伤口愈合的损害作用，过度使用 TPM 可能导致慢性伤口和口腔溃疡的产生。
