Biosafety Research Institute, Chonbuk National University, Jeonju, South Korea.
Environ Toxicol Pharmacol. 2006 May;21(3):308-16. doi: 10.1016/j.etap.2005.10.002. Epub 2005 Dec 5.
Wound angiogenesis is essential to support the regenerating tissue and any setback in angiogenesis may result in retarded wound repair. Cigarette smoking causes numerous adverse effects, some of which are associated with poor healing. The current experiment was carried out to elucidate the possible detrimental effects of sidestream whole smoke solutions (SSWSS) on wound healing and related angiogenesis, using a well-defined chicken dorsum excision wound assay. Gross, histopathologic, SEM and computer based 3D image-probing modalities were utilized to quantify different detrimental effects of SSWSS on the fundamental processes of wound healing. A total of 160 chicks, aged 1 week, divided in eight groups were topically exposed for 8 days to SSWSS with different nicotine concentrations. At day 6 and day 8 post-wounding, very highly significant reduction (P<0.001) in wound closure was observed among all SSWSS treated groups. Histological and SEM evaluation of SSWSS treated wounds unveiled deteriorated dermal matrix, delayed re-epithelialization and retarded neovascularization. Moreover, image-probing exploration of SSWSS treated wounds also divulge a very highly significant decrease (P<0.001) in angular spectrum, Sa, Sy and Sci, at day 6 post-wounding. Our study suggests that the cumulative effect of different components of SSWSS has a negative impact on wound healing and related angiogenesis. Furthermore, our study demonstrates the effects that can contribute to abnormal healing and may explain why people who are consistently exposed to sidestream smoke suffer from slow healing and excessive scarring of wounds, much like the smokers themselves.
伤口血管生成对于支持再生组织至关重要，任何血管生成的延迟都可能导致伤口修复迟缓。吸烟会引起许多不良反应，其中一些与愈合不良有关。本实验旨在利用明确的鸡背部切除伤口模型，阐明侧流全烟溶液（SSWSS）对伤口愈合和相关血管生成的可能有害影响。使用大体、组织病理学、SEM 和基于计算机的 3D 图像探测方法来量化 SSWSS 对伤口愈合基本过程的不同有害影响。将 160 只 1 周龄的小鸡分为 8 组，每天接受不同尼古丁浓度的 SSWSS 局部暴露 8 天。在创伤后第 6 天和第 8 天，所有 SSWSS 处理组的伤口闭合率均显著降低（P<0.001）。对 SSWSS 处理伤口的组织学和 SEM 评估显示，真皮基质恶化，上皮再形成延迟，新生血管形成迟缓。此外，对 SSWSS 处理伤口的图像探测探测也显示，在创伤后第 6 天，角谱、Sa、Sy 和 Sci 非常显著降低（P<0.001）。我们的研究表明，SSWSS 的不同成分的累积效应对伤口愈合和相关血管生成有负面影响。此外，我们的研究还证明了这些效应可能导致异常愈合，并解释了为什么经常暴露于侧流烟雾的人会出现伤口愈合缓慢和过度瘢痕形成等问题，就像吸烟者自己一样。
