Department of Biochemistry & Biotechnology, Annamalai University, Annamalainagar 608002, Tamilnadu, India.
Environ Toxicol Pharmacol. 2009 Sep;28(2):192-7. doi: 10.1016/j.etap.2009.04.003. Epub 2009 Apr 11.
Sesamol (SM) is a dietary phytochemical present in the processed sesame oil. In this present study we have evaluated the antioxidant potential of SM and its role in the protection of radiation-induced DNA damage in γ-irradiated mice. The antioxidant properties of SM were evaluated by using different in vitro antioxidant assays. SM shows scavenging effect against hydroxyl (OH), superoxide anion (O(2)(-)), nitric oxide, 2,2-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid radical cation (ABTS(+)) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. Our results demonstrate that SM exhibits strong antioxidant property in all the in vitro assays. When mice were exposed to 7 Gy γ-radiations there was an increase in % tail DNA, tail length, tail moment and Olive tail moment in blood lymphocytes. SM (100mg/kg b.wt) pretreatment significantly decreased the % tail DNA, tail length, tail moment and Olive tail moment in irradiated mice lymphocytes. These results suggest that SM protects γ-radiation-induced DNA damage in mice lymphocytes, which may be attributed to its antioxidant property.
芝麻酚（SM）是加工芝麻油中的一种膳食植物化学物质。在本研究中，我们评估了 SM 的抗氧化潜力及其在保护γ-辐照小鼠辐射诱导的 DNA 损伤中的作用。通过使用不同的体外抗氧化测定法评估了 SM 的抗氧化特性。SM 对羟基（OH）、超氧阴离子（O(2)(-））、一氧化氮、2,2-偶氮-双-3-乙基苯并噻唑啉-6-磺酸自由基阳离子（ABTS(+））和 1,1-二苯基-2-苦基肼（DPPH）自由基具有清除作用。我们的结果表明，SM 在所有体外测定中均表现出很强的抗氧化特性。当小鼠暴露于 7 Gy γ 射线时，血液淋巴细胞中的%尾 DNA、尾长、尾矩和 Olive 尾矩增加。SM（100mg/kg b.wt）预处理可显著降低辐照小鼠淋巴细胞中的%尾 DNA、尾长、尾矩和 Olive 尾矩。这些结果表明，SM 可保护小鼠淋巴细胞中 γ 辐射诱导的 DNA 损伤，这可能归因于其抗氧化特性。
