INIBIOLP (Instituto de Investigaciones Bioquímicas de La Plata), CCT La Plata, CONICET-UNLP, Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, Calles 60 y 120, 1900 La Plata, Argentina.
Environ Toxicol Pharmacol. 2009 Nov;28(3):465-73. doi: 10.1016/j.etap.2009.07.009. Epub 2009 Aug 7.
The effect of dimethoate, zineb and glyphosate administered alone or in combination on liver, kidney, brain and plasma antioxidant defense system was investigated. Lipid peroxidation, and RNS production were increased in all tissues studied, especially in those groups that received a combination of drugs. Intoxicated rats exhibited lower antioxidant ability, higher oxidized protein and glutathione levels in plasma with a decreased concentration of α-tocopherol in brain and liver, between 30% and 60% of control. Superoxide dismutase was decreased in liver and brain. Glutathione reductase was inhibited in liver while glutathione peroxidase and transferase were unaffected. Plasma lactate dehydrogenase and γ-glutamyl transpeptidase activities were both increased. The associations of drugs produce more damage than individual administration being the effects observed strongly dependent on the kind of tissue analyzed. In conclusion, the present paper evidenced both the role of the oxidative stress as a mechanism of action of some pesticides and the potential additive effects of a simultaneous exposure to more than one compound. In addition, results suggest a potential contribution of pesticide mixtures to the aetiology of some neurodegenerative diseases.
单独或联合使用乐果、代森锌和草甘膦对肝脏、肾脏、大脑和血浆抗氧化防御系统的影响。所有研究组织的脂质过氧化和 RNS 产生均增加，尤其是接受联合用药的组。中毒大鼠表现出较低的抗氧化能力，血浆中氧化蛋白和谷胱甘肽水平升高，大脑和肝脏中 α-生育酚浓度降低 30%至 60%。肝和脑中的超氧化物歧化酶减少。肝中谷胱甘肽还原酶受到抑制，而谷胱甘肽过氧化物酶和转移酶不受影响。血浆乳酸脱氢酶和γ-谷氨酰转肽酶活性均升高。药物的联合作用比单独给药产生更多的损害，观察到的影响强烈依赖于所分析的组织类型。总之，本研究证明了氧化应激作为一些农药作用机制的作用，以及同时接触多种化合物的潜在附加作用。此外，结果表明农药混合物可能对某些神经退行性疾病的病因有一定的贡献。
