Sunnybrook Research Institute, Sunnybrook Health Sciences Centre, Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada.
FEBS Lett. 2011 Aug 19;585(16):2610-6. doi: 10.1016/j.febslet.2011.07.016. Epub 2011 Jul 23.
We investigated the roles of the non-coding transcripts and found that expression of a fragment containing the 3'-untranslated region (3'-UTR) of nephronectin in osteoblast progenitor cells MC3T3-E1 promoted cell differentiation dramatically. We hypothesized that the ectopically expressed 3'-UTR binds microRNAs and modulates their functions. β-Catenin and GSK3β were up-regulated in the 3'-UTR-transfected cells, contributing to the increased cell differentiation, through reduction of EGFR and ERK phosphorylation. Activator of GSK3β promoted differentiation, while inhibitor of GSK3β blocked differentiation. Our results indicate that the non-coding transcripts are important in regulating cell activities and may have potential application for modulating endogenous microRNA functions.
我们研究了非编码转录本的作用，发现含有纤连蛋白 3’-非翻译区（3’-UTR）片段的表达在成骨前体细胞 MC3T3-E1 中显著促进细胞分化。我们假设异位表达的 3’-UTR 结合 microRNAs 并调节它们的功能。β-连环蛋白和 GSK3β 在 3’-UTR 转染细胞中上调，通过减少 EGFR 和 ERK 磷酸化，促进细胞分化。GSK3β 的激活剂促进分化，而 GSK3β 的抑制剂则阻止分化。我们的结果表明，非编码转录本在调节细胞活性方面起着重要作用，并且可能具有调节内源性 microRNA 功能的潜在应用。
