Ikehata Mikiko, Yamada Atsushi, Morimura Naoko, Itose Masakatsu, Suzawa Tetsuo, Shirota Tatsuo, Chikazu Daichi, Kamijo Ryutaro
Department of Biochemistry, School of Dentistry, Showa University, Tokyo, Japan; Department of Oral and Maxillofacial Surgery, Tokyo Medical University, Tokyo, Japan.
Department of Biochemistry, School of Dentistry, Showa University, Tokyo, Japan.
Biochem Biophys Res Commun. 2017 Mar 4;484(2):231-234. doi: 10.1016/j.bbrc.2017.01.053. Epub 2017 Jan 16.
Nephronectin (Npnt), an extracellular matrix protein, is considered to play critical roles as an adhesion molecule in the development and functions of various organs and tissues, such as the kidneys and bone. In the present study, we found that Wnt3a strongly enhanced Npnt mRNA expression in osteoblast-like MC3T3-E1 cells, while it also induced an increase in Npnt gene expression in both time- and dose-dependent manners via the Wnt/β-catenin signaling pathway. These results suggest novel mechanisms for Wnt3a-induced osteoblast proliferation and cell survival via Npnt gene expression.
肾连蛋白（Npnt）是一种细胞外基质蛋白，被认为作为一种黏附分子在包括肾脏和骨骼在内的各种器官和组织的发育及功能中发挥关键作用。在本研究中，我们发现Wnt3a强烈增强成骨样MC3T3-E1细胞中Npnt mRNA的表达，同时它还通过Wnt/β-连环蛋白信号通路以时间和剂量依赖的方式诱导Npnt基因表达增加。这些结果提示了Wnt3a通过Npnt基因表达诱导成骨细胞增殖和细胞存活的新机制。
