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纤连蛋白通过表皮生长因子样重复序列促进成骨细胞分化。

Nephronectin promotes osteoblast differentiation via the epidermal growth factor-like repeats.

机构信息

Sunnybrook Research Institute, Sunnybrook Health Sciences Centre, Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Canada.

出版信息

FEBS Lett. 2010 Jan 4;584(1):233-8. doi: 10.1016/j.febslet.2009.11.077.

DOI:10.1016/j.febslet.2009.11.077
PMID:19944102
Abstract

We found that nephronectin was significantly down-regulated by TGF-beta1. To determine the function of nephronectin in osteogenesis, we generated various constructs to produce stable MC3T3-E1 cell lines, expressing and secreting nephronectin protein, including full-length (Npnt), lacking EGF-like repeats (Np-MAM), and lacking RGD and MAM domains (Np-EGF). We demonstrated that nephronectin promotes differentiation during osteoblast differentiation and the EGF-like repeats were essential. Lack of these repeats resulted in inhibiting the change in morphology. Over-expression of nephronectin results in earlier formation of bone nodules than the vector control. ERK activation is essential for nephronectin-induced osteoblast differentiation.

摘要

我们发现,nephronectin 受 TGF-β1 显著下调。为了确定 nephronectin 在成骨中的功能,我们构建了各种表达载体,使 MC3T3-E1 细胞系稳定表达并分泌 nephronectin 蛋白,包括全长(Npnt)、缺乏 EGF 样重复(Np-MAM)和缺乏 RGD 和 MAM 结构域(Np-EGF)。我们证明 nephronectin 促进成骨细胞分化,EGF 样重复是必需的。缺乏这些重复会导致形态改变受到抑制。nephronectin 的过表达导致骨结节的形成早于载体对照。ERK 激活对于 nephronectin 诱导的成骨细胞分化是必需的。

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FEBS Lett. 2010 Jan 4;584(1):233-8. doi: 10.1016/j.febslet.2009.11.077.
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