INEB - Instituto de Engenharia Biomédica, Rua do Campo Alegre, n° 823, 4150-180 Porto, Portugal.
Biomaterials. 2011 Nov;32(31):7897-904. doi: 10.1016/j.biomaterials.2011.07.013. Epub 2011 Jul 23.
Cell-based therapies offer an attractive approach for revascularization and regeneration of tissues. However, and despite the pressing clinical needs for effective revascularization strategies, the successful immobilization of viable vascular cells within 3D matrices has been difficult to achieve. In this paper the in vitro potential of a natural, injectable RGD-alginate hydrogel as an in situ forming matrix to deliver endothelial cells was evaluated. Several techniques were employed to investigate how these microenvironments could influence the behavior of vascular cells, namely their ability to promote the outward migration of viable, proliferative cells, retaining the ability to form a 3D arrangement. Cells within RGD-grafted alginate hydrogel were able to proliferate and maintained 80% of viability for at least 48 h post-immobilization. Additionally, entrapped cells created a 3D organization into cellular networks and, when put in contact with matrigel, cells migrated out of the RGD-matrix. Overall, the obtained results support the idea that the RGD peptides conjugated to alginate provide a 3D environment for endothelial cells adhesion, survival, migration and organization.
细胞疗法为组织的再血管化和再生提供了一种有吸引力的方法。然而，尽管临床上迫切需要有效的再血管化策略，但将有活力的血管细胞成功固定在 3D 基质中一直难以实现。本文评估了一种天然可注射的 RGD-藻酸盐水凝胶作为原位形成基质用于输送内皮细胞的体外潜力。采用了多种技术来研究这些微环境如何影响血管细胞的行为，即它们促进有活力的增殖细胞向外迁移的能力，同时保持形成 3D 排列的能力。在 RGD 接枝藻酸盐水凝胶中的细胞能够增殖，并在固定后至少 48 小时内保持 80%的活力。此外，包埋的细胞形成了细胞网络的 3D 组织，并且当与基质胶接触时，细胞从 RGD 基质中迁移出来。总的来说，所得结果支持这样的观点，即与藻酸盐共轭的 RGD 肽为内皮细胞的黏附、存活、迁移和组织提供了 3D 环境。
