Division of Endodontics and Operative Dentistry, Department of Restorative and Biomaterials Sciences, Meikai University School of Dentistry, Saitama, Japan;
Division of Endodontics and Operative Dentistry, Department of Restorative and Biomaterials Sciences, Meikai University School of Dentistry, Saitama, Japan.
In Vivo. 2021 Jan-Feb;35(1):313-317. doi: 10.21873/invivo.12261.
Enamel matrix derivative (EMD) is widely used for regeneration therapy in dental clinical situations, but the mechanism of EMD bioactivity remains obscure. To clarify this mechanism, we focused on the formation of connective tissue and blood vessels. The aim of this study was to confirm whether EMD induces the formation of connective tissue and blood vessels by using the diffusion chamber (DC) technique.
Individual DCs containing EMD (DC-EMD) or propylene glycol alginate (PGA) were implanted subcutaneously in rat dorsum. At 4 weeks after the implantation, histological analysis of DCs was performed using azan staining.
DC-EMD induced the formation of much larger amounts of connective tissue containing abundant blood vessels than did DC-PGA.
The results indicated that EMD can induce the formation of both connective tissue and blood vessels. This bioactivity may contribute to the mechanism whereby EMD induces tissue regeneration.
釉基质衍生物（EMD）在口腔临床再生治疗中被广泛应用，但 EMD 的生物活性机制仍不清楚。为了阐明这一机制，我们重点关注了结缔组织和血管的形成。本研究旨在使用扩散室（DC）技术证实 EMD 是否能诱导结缔组织和血管的形成。
将含有 EMD（DC-EMD）或藻酸丙二醇酯（PGA）的单个 DC 植入大鼠背部皮下。植入后 4 周，通过偶氮染色对 DC 进行组织学分析。
DC-EMD 诱导形成的含有丰富血管的结缔组织明显多于 DC-PGA。
结果表明，EMD 可诱导结缔组织和血管的形成。这种生物活性可能有助于解释 EMD 诱导组织再生的机制。
