基于基因表达和活性测定的纤维小体蛋白酶抑制剂在纤维梭菌中的推测作用。
Putative role of cellulosomal protease inhibitors in Clostridium cellulovorans based on gene expression and measurement of activities.
机构信息
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo, Kyoto 606-8502, Japan.
出版信息
J Bacteriol. 2011 Oct;193(19):5527-30. doi: 10.1128/JB.05022-11. Epub 2011 Jul 22.
Abstract
This study is the first to demonstrate the activity of putative cellulosomal protease/peptidase inhibitors (named cyspins) of Clostridium cellulovorans, using the Saccharomyces cerevisiae display system. Cyspins exhibited inhibitory activities against several representative plant proteases. This suggests that these inhibitors protect their microbe and cellulosome from external attack by plant proteases.
摘要
这项研究首次利用酿酒酵母展示系统证明了纤维素分解梭菌（Clostridium cellulovorans）假定的纤维小体蛋白酶/肽酶抑制剂（命名为 cyspin）的活性。Cyspin 对几种代表性的植物蛋白酶表现出抑制活性。这表明这些抑制剂可以保护微生物及其纤维小体免受植物蛋白酶的外部攻击。
相似文献
1
Putative role of cellulosomal protease inhibitors in Clostridium cellulovorans based on gene expression and measurement of activities.基于基因表达和活性测定的纤维小体蛋白酶抑制剂在纤维梭菌中的推测作用。
J Bacteriol. 2011 Oct;193(19):5527-30. doi: 10.1128/JB.05022-11. Epub 2011 Jul 22.
2
Temporal proteome dynamics of Clostridium cellulovorans cultured with major plant cell wall polysaccharides.梭菌属纤维单胞菌利用主要植物细胞壁多糖培养的时间蛋白质组动态变化。
BMC Microbiol. 2019 Jun 3;19(1):118. doi: 10.1186/s12866-019-1480-0.
3
Genome sequence of the cellulosome-producing mesophilic organism Clostridium cellulovorans 743B.产纤维素酶的中温微生物梭菌 743B 的基因组序列。
J Bacteriol. 2010 Feb;192(3):901-2. doi: 10.1128/JB.01450-09. Epub 2009 Nov 30.
4
Cellulosomic profiling produced by Clostridium cellulovorans during growth on different carbon sources explored by the cohesin marker.通过黏连蛋白标记物对不同碳源上生长的纤维梭菌的细胞酶谱进行分析。
J Biotechnol. 2010 Feb 1;145(3):233-9. doi: 10.1016/j.jbiotec.2009.11.020. Epub 2009 Dec 1.
5
Effect of carbon source on the cellulosomal subpopulations of Clostridium cellulovorans.碳源对食纤维梭菌纤维小体亚群的影响。
Microbiology (Reading). 2005 May;151(Pt 5):1491-1497. doi: 10.1099/mic.0.27605-0.
6
Exoproteome profiles of Clostridium cellulovorans grown on various carbon sources.不同碳源培养的纤维梭菌外蛋白质组图谱。
Appl Environ Microbiol. 2013 Nov;79(21):6576-84. doi: 10.1128/AEM.02137-13. Epub 2013 Aug 16.
7
Comparative genomics of the mesophilic cellulosome-producing Clostridium cellulovorans and its application to biofuel production via consolidated bioprocessing.嗜温产纤维素酶梭菌的比较基因组学及其在通过整合生物加工生产生物燃料中的应用。
Environ Technol. 2010 Jul-Aug;31(8-9):889-903. doi: 10.1080/09593330.2010.490856.
8
Degradation of cellulosome-produced cello-oligosaccharides by an extracellular non-cellulosomal beta-glucan glucohydrolase, BglA, from Clostridium cellulovorans.来自食纤维梭菌的一种胞外非纤维小体β-葡聚糖葡萄糖水解酶BglA对纤维小体产生的纤维寡糖的降解作用
Biochem Biophys Res Commun. 2006 Oct 13;349(1):20-3. doi: 10.1016/j.bbrc.2006.07.038. Epub 2006 Jul 17.
9
Comparison of the mesophilic cellulosome-producing Clostridium cellulovorans genome with other cellulosome-related clostridial genomes.比较嗜温产纤维素酶梭菌（Clostridium cellulovorans）基因组与其他与纤维素酶相关的梭菌基因组。
Microb Biotechnol. 2011 Jan;4(1):64-73. doi: 10.1111/j.1751-7915.2010.00210.x.
10
Synthesis of Clostridium cellulovorans minicellulosomes by intercellular complementation.通过细胞间互补合成嗜纤维梭菌微小纤维素体
Proc Natl Acad Sci U S A. 2007 Jan 30;104(5):1456-60. doi: 10.1073/pnas.0610740104. Epub 2007 Jan 23.
引用本文的文献
1
A cellulosomal double-dockerin module from Clostridium thermocellum shows distinct structural and cohesin-binding features.热纤梭菌的纤维素酶系双 dockerin 模块具有独特的结构和黏附素结合特征。
Protein Sci. 2024 Apr;33(4):e4937. doi: 10.1002/pro.4937.
2
Temporal proteome dynamics of Clostridium cellulovorans cultured with major plant cell wall polysaccharides.梭菌属纤维单胞菌利用主要植物细胞壁多糖培养的时间蛋白质组动态变化。
BMC Microbiol. 2019 Jun 3;19(1):118. doi: 10.1186/s12866-019-1480-0.
3
Exoproteome analysis of in natural soft-biomass degradation.天然软生物质降解过程中的胞外蛋白质组分析。
AMB Express. 2015 Jan 24;5(1):2. doi: 10.1186/s13568-014-0089-9. eCollection 2015 Dec.
4
Exoproteome profiles of Clostridium cellulovorans grown on various carbon sources.不同碳源培养的纤维梭菌外蛋白质组图谱。
Appl Environ Microbiol. 2013 Nov;79(21):6576-84. doi: 10.1128/AEM.02137-13. Epub 2013 Aug 16.
5
Extending the cellulosome paradigm: the modular Clostridium thermocellum cellulosomal serpin PinA is a broad-spectrum inhibitor of subtilisin-like proteases.扩展细胞外酶体系范例：模块化梭菌热纤维梭菌细胞外酶体系丝氨酸蛋白酶抑制剂 PinA 是一种广谱的枯草杆菌蛋白酶样蛋白酶抑制剂。
Appl Environ Microbiol. 2013 Oct;79(19):6173-5. doi: 10.1128/AEM.01912-13. Epub 2013 Jul 19.
6
Profile of native cellulosomal proteins of Clostridium cellulovorans adapted to various carbon sources.适应不同碳源的纤维梭菌天然细胞外纤维蛋白复合体蛋白的特性。
AMB Express. 2012 Jul 29;2(1):37. doi: 10.1186/2191-0855-2-37.
本文引用的文献
1
Comparative genomics of the mesophilic cellulosome-producing Clostridium cellulovorans and its application to biofuel production via consolidated bioprocessing.嗜温产纤维素酶梭菌的比较基因组学及其在通过整合生物加工生产生物燃料中的应用。
Environ Technol. 2010 Jul-Aug;31(8-9):889-903. doi: 10.1080/09593330.2010.490856.
2
Genome sequence of the cellulosome-producing mesophilic organism Clostridium cellulovorans 743B.产纤维素酶的中温微生物梭菌 743B 的基因组序列。
J Bacteriol. 2010 Feb;192(3):901-2. doi: 10.1128/JB.01450-09. Epub 2009 Nov 30.
3
The cellulosomes from Clostridium cellulolyticum: identification of new components and synergies between complexes.解纤维梭菌的纤维小体：新组分的鉴定及复合物间的协同作用
FEBS J. 2009 Jun;276(11):3076-86. doi: 10.1111/j.1742-4658.2009.07025.x. Epub 2009 Apr 22.
4
Enhancement of display efficiency in yeast display system by vector engineering and gene disruption.通过载体工程和基因破坏提高酵母展示系统中的展示效率。
Appl Microbiol Biotechnol. 2009 Mar;82(4):713-9. doi: 10.1007/s00253-008-1808-4. Epub 2009 Jan 3.
5
A serpin in the cellulosome of the anaerobic fungus Piromyces sp. strain E2.厌氧真菌梨形毛霉属菌株E2的纤维小体中的一种丝氨酸蛋白酶抑制剂。
Mycol Res. 2008 Aug;112(Pt 8):999-1006. doi: 10.1016/j.mycres.2008.01.021. Epub 2008 Feb 16.
6
Occurrence and properties of proteases in plant latices.植物乳汁中蛋白酶的存在与特性
Planta Med. 2008 Jun;74(7):699-711. doi: 10.1055/s-2008-1074530. Epub 2008 May 21.
7
Protease gene families in Populus and Arabidopsis.杨树和拟南芥中的蛋白酶基因家族。
BMC Plant Biol. 2006 Dec 20;6:30. doi: 10.1186/1471-2229-6-30.
8
The functional repertoire of prokaryote cellulosomes includes the serpin superfamily of serine proteinase inhibitors.原核生物纤维小体的功能组成包括丝氨酸蛋白酶抑制剂的丝氨酸蛋白酶抑制剂超家族。
Mol Microbiol. 2006 Jun;60(6):1344-54. doi: 10.1111/j.1365-2958.2006.05182.x.
9
Protease inhibitors in bacteria: an emerging concept for the regulation of bacterial protein complexes?细菌中的蛋白酶抑制剂：调控细菌蛋白质复合物的一个新兴概念？
Mol Microbiol. 2006 Jun;60(6):1323-6. doi: 10.1111/j.1365-2958.2006.05181.x.
10
Isolation and Characterization of an Anaerobic, Cellulolytic Bacterium, Clostridium cellulovorans sp. nov.一株厌氧产纤维素菌——纤维梭菌的分离与鉴定
Appl Environ Microbiol. 1984 Jul;48(1):88-93. doi: 10.1128/aem.48.1.88-93.1984.
Zotero 插件