通过细胞间互补合成嗜纤维梭菌微小纤维素体
Synthesis of Clostridium cellulovorans minicellulosomes by intercellular complementation.
作者信息
Arai Takamitsu, Matsuoka Satoshi, Cho Hee-Yeon, Yukawa Hideaki, Inui Masayuki, Wong Sui-Lam, Doi Roy H
机构信息
Section of Molecular and Cellular Biology, University of California, Davis, CA 95616-8535, USA.
出版信息
Proc Natl Acad Sci U S A. 2007 Jan 30;104(5):1456-60. doi: 10.1073/pnas.0610740104. Epub 2007 Jan 23.
Abstract
The ability of two strains of bacteria to cooperate in the synthesis of an enzyme complex (a minicellulosome) was examined. Three strains of Bacillus subtilis were constructed to express Clostridium cellulovorans genes engB, xynB, and minicbpA. MiniCbpA, EngB, and XynB were synthesized and secreted into the medium by B. subtilis. When the strains with the minicbpA and engB genes or with xynB were cocultured, minicellulosomes were synthesized, consisting in one case of miniCbpA and EngB and in the second case of miniCbpA and XynB. Both minicellulosomes showed their respective enzymatic activities. We call this phenomenon "intercellular complementation." Interesting implications concerning bacterial cooperation are suggested from these results.
摘要
研究了两株细菌在合成酶复合物（一种小型纤维小体）方面的合作能力。构建了三株枯草芽孢杆菌菌株来表达食纤维梭菌的engB、xynB和minicbpA基因。枯草芽孢杆菌合成并分泌了MiniCbpA、EngB和XynB到培养基中。当携带minicbpA和engB基因的菌株或携带xynB基因的菌株共培养时，合成了小型纤维小体，一种情况是由MiniCbpA和EngB组成，另一种情况是由MiniCbpA和XynB组成。两种小型纤维小体都表现出各自的酶活性。我们将这种现象称为“细胞间互补”。这些结果暗示了有关细菌合作的有趣含义。
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