Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Int J Syst Evol Microbiol. 2012 Jun;62(Pt 6):1284-1288. doi: 10.1099/ijs.0.032102-0. Epub 2011 Jul 22.
A Gram-positive, rod-shaped, xylanolytic, spore-forming bacterium, strain GTH-3(T), was isolated from a tidal flat adjacent to Ganghwa Island, Republic of Korea, and was characterized to determine its taxonomic position. On the basis of 16S rRNA gene sequence similarity, strain GTH-3(T) was shown to belong to the family Paenibacillaceae, being most closely related to the type strains of Paenibacillus ginsengisoli (94.9 %), Paenibacillus anaericanus (94.8 %), Paenibacillus urinalis (94.4 %), Paenibacillus cookii (94.2 %), Paenibacillus alvei (94.1 %) and Paenibacillus chibensis (94.0 %). The G+C content of the genomic DNA of strain GTH-3(T) was 45.9±0.2 mol% (mean±sd). The major menaquinone was MK-7. The major fatty acids were anteiso-C(15:0) and iso-C(16:0). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Phenotypic and chemotaxonomic data supported the affiliation of strain GTH-3(T) to the genus Paenibacillus. The results of physiological and biochemical tests allowed strain GTH-3(T) to be distinguished genotypically and phenotypically from recognized species of the genus Paenibacillus. Strain GTH-3(T) is therefore considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus sediminis sp. nov. is proposed. The type strain is GTH-3(T) ( = DSM 23491(T) = LMG 25635(T)).
一株革兰氏阳性、杆状、分解木聚糖、产孢子的细菌，菌株 GTH-3(T)，从韩国 Ganghwa 岛附近的潮滩中分离得到，并对其进行了特征描述以确定其分类地位。根据 16S rRNA 基因序列相似性，菌株 GTH-3(T)属于芽孢杆菌科，与人参芽孢杆菌（94.9%）、厌氧芽孢杆菌（94.8%）、尿芽孢杆菌（94.4%）、库克芽孢杆菌（94.2%）、短小芽孢杆菌（94.1%）和中华芽孢杆菌（94.0%）的模式菌株最为密切相关。菌株 GTH-3(T)的基因组 DNA G+C 含量为 45.9±0.2 mol%（平均值±标准差）。主要的menaquinone 是 MK-7。主要脂肪酸是 anteiso-C(15:0)和 iso-C(16:0)。主要的极性脂质是双磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺。表型和化学分类学数据支持菌株 GTH-3(T)属于芽孢杆菌属。生理生化试验的结果使菌株 GTH-3(T)在基因型和表型上与芽孢杆菌属的公认种区分开来。因此，菌株 GTH-3(T)被认为代表了芽孢杆菌属的一个新种，建议将其命名为 sediminis 芽孢杆菌 sp. nov.。其模式菌株是 GTH-3(T)（=DSM 23491(T)=LMG 25635(T)）。
