Manchester Royal Eye Hospital, Manchester, UK.
Br J Ophthalmol. 2011 Oct;95(10):1463-5. doi: 10.1136/bjophthalmol-2011-300360. Epub 2011 Jul 23.
To determine if residual corneal stroma remains on the recipient posterior lamella in big-bubble deep anterior lamellar keratoplasty (DALK).
Pneumodissection using the big-bubble technique was carried out on eye-bank corneas mounted on an artificial anterior chamber. Samples that had a successful big-bubble formation were sent for histological evaluation to determine if any residual stroma remained on the Descemet membrane (DM).
Big-bubble formation was achieved in 32 donor corneas. Two distinct types of big-bubble were seen: the bubble had either a white margin (30 corneas) or a clear margin (two corneas). The posterior lamellae of all the white margin corneas showed residual stroma on DM with a mean central thickness of 7.0 μm (range 2.6-17.4 μm). The clear margin corneas showed no residual stroma on DM.
It should no longer be assumed that big-bubble DALK, where the bubble has a white margin, routinely bares DM. True baring of DM may only occur with the less commonly seen clear margin bubble.
确定在大泡深板层角膜切开术（DALK）中，受者后板层的角膜基质是否有残留。
在 mounted on an artificial anterior chamber 的眼库角膜上进行气动分离，使用大泡技术。对成功形成大泡的样本进行组织学评估，以确定在 Descemet 膜（DM）上是否有任何残留的基质。
在 32 个供体角膜中成功形成了大泡。两种不同类型的大泡：泡有白色边缘（30 个角膜）或透明边缘（2 个角膜）。所有白色边缘角膜的后板层在 DM 上均显示残留的基质，平均中央厚度为 7.0 μm（范围 2.6-17.4 μm）。透明边缘的角膜在 DM 上没有残留的基质。
不应再假设大泡 DALK，其中泡有白色边缘，常规地暴露 DM。真正的 DM 暴露可能仅发生在不太常见的透明边缘泡中。
