Feizi Sepehr, Kanavi Mozhgan Rezaei, Kharaghani Davood, Balagholi Sahar, Meskinfam Masoumeh, Javadi Mohammad Ali
Ophthalmic Research Center, Labbafinejad Medical Center, Shahid Beheshti University of Medical Sciences, Boostan 9 St., Pasdaran Ave., Tehran, 16666, Iran.
Ocular Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Jpn J Ophthalmol. 2016 Nov;60(6):492-499. doi: 10.1007/s10384-016-0465-x. Epub 2016 Jul 19.
To characterize the walls of big bubbles formed during deep anterior lamellar keratoplasty (DALK) using the corrosion casting technique.
Fresh corneoscleral buttons with normal transparency and without any known eye diseases (n = 11) were obtained from 11 human donors. A 20-gauge needle was used to inject a solution of 20 % polyvinyl alcohol (PVA) immediately beneath the corneal endothelium to form big bubbles in eight corneoscleral buttons. In the second experiment on three corneoscleral buttons, a big bubble was first formed by air injection beneath the endothelium. Thereafter, 20 % PVA was injected into the bubble space. Scanning electron microscopy was used to characterize the surfaces of the casts, which replicated the walls of the big bubbles.
A type-1 bubble was formed in all corneas. In one cornea, one type-1 bubble was initially formed centrally, and while it was enlarged, an eccentric type-2 bubble appeared. Scanning electron microscopy showed that the casts of type-1 bubbles had two distinct surfaces. The anterior surface demonstrated several holes or pits, depending on the material used for the bubble formation, whereas the posterior surface exhibited an uneven surface. The anterior and posterior surfaces of the type-2 cast were more or less similar. A communication measuring 531.9 µm in length and 171.4 µm in diameter was found between the two bubbles.
The corrosion casting technique provides a permanent three-dimensional record of the potential spaces and barriers in the posterior corneal stroma, which explains several features associated with big-bubble DALK.
使用腐蚀铸型技术对深层前板层角膜移植术（DALK）过程中形成的大气泡壁进行特征描述。
从11名人类供体获取11个具有正常透明度且无任何已知眼部疾病的新鲜角膜缘植片。使用20号针头在8个角膜缘植片的角膜内皮下方立即注射20%聚乙烯醇（PVA）溶液以形成大气泡。在对3个角膜缘植片进行的第二个实验中，首先通过在内皮下方注射空气形成一个大气泡。此后，将20%PVA注入气泡空间。使用扫描电子显微镜对铸型表面进行特征描述，铸型复制了大气泡的壁。
所有角膜均形成了1型气泡。在一个角膜中，最初在中央形成了一个1型气泡，在其扩大时，出现了一个偏心的2型气泡。扫描电子显微镜显示，1型气泡的铸型有两个不同的表面。前表面根据用于形成气泡的材料显示出几个孔或凹坑，而后表面呈现出不平整的表面。2型铸型的前表面和后表面或多或少相似。在两个气泡之间发现了一个长度为531.9μm、直径为171.4μm的连通通道。
腐蚀铸型技术提供了后角膜基质中潜在空间和屏障的永久性三维记录，这解释了与大气泡DALK相关的几个特征。
