Department of Oto-Rhino-Laryngology, Head- and Neck Surgery, Semmelweis University, Szigony u. 36, Budapest, 1083, Hungary.
Eur Arch Otorhinolaryngol. 2012 Feb;269(2):513-21. doi: 10.1007/s00405-011-1710-z. Epub 2011 Jul 23.
Our aim was to study the association of Pro12Ala and exon6 C161T polymorphisms of PPARgamma and intron7 G/C polymorphisms of PPAR-alpha with clinical symptoms, peak nasal inspiratory flow values, serum soluble TNF-alpha, TNF-R1, Fas, Fas ligand and IgE concentrations in patients with seasonal allergic rhinitis during and after pollen season. We performed a follow-up study of 66 Hungarian patients with seasonal allergic rhinitis and 180 healthy referent subjects. We used PCR-RFLP technique and ELISA. The distribution of mutant alleles of PPAR-gamma and -alpha did not differ in patients and referent subjects. Patients carrying the mutant 12Ala, exon6 161T alleles of PPAR-gamma and intron7 C allele of PPAR-alpha had significantly higher clinical symptom score values, TNF-alpha and IgE levels and lower peak nasal inspiratory flow values during and after pollen season. The results indicated that nuclear receptors PPAR-gamma and PPAR-alpha are involved in the regulation of inflammatory mediator production in patients with seasonal allergic rhinitis and polymorphisms of the receptors are very likely to contribute to the heterogeneity of clinical and immunological parameters of allergic patients.
我们的目的是研究过氧化物酶体增殖物激活受体γ（PPARγ）基因 Pro12Ala 和外显子 6 C161T 多态性、PPAR-α 基因内含子 7 G/C 多态性与花粉季节期间和之后季节性变应性鼻炎患者的临床症状、峰流速值、血清可溶性肿瘤坏死因子-α（TNF-α）、TNF 受体 1（TNF-R1）、Fas、Fas 配体和 IgE 浓度之间的相关性。我们对 66 名匈牙利季节性变应性鼻炎患者和 180 名健康对照者进行了随访研究。我们使用 PCR-RFLP 技术和 ELISA。PPAR-γ 和 -α 基因的突变等位基因在患者和对照者中的分布没有差异。携带 PPAR-γ 12Ala 突变等位基因、外显子 6 161T 等位基因和 PPAR-α 内含子 7 C 等位基因的患者在花粉季节期间和之后具有更高的临床症状评分值、TNF-α 和 IgE 水平以及更低的峰流速值。结果表明，核受体 PPAR-γ 和 PPAR-α 参与调节季节性变应性鼻炎患者的炎症介质产生，受体的多态性很可能导致过敏患者的临床和免疫学参数的异质性。
