Zakrzewska Anna, Gryczyńska Danuta, Kobos Józef, Górski Paweł
Department of Pediatric Otolaryngology, Medical University of Lodz, Lodz, Poland.
Int Arch Allergy Immunol. 2006;140(3):223-30. doi: 10.1159/000093247. Epub 2006 May 9.
The balance of CD28/CTLA4-derived signals and Fas-dependent apoptosis activity is determined by the peripheral defense mechanisms and might play a role in the pathogenesis of allergy.
The aim of the study was to investigate the expression of costimulatory and pro- and antiapoptotic molecules in adenoid T cells of children suffering from allergic rhinitis and to find out which of these molecules have a predictive value in the development of allergic rhinitis.
The adenoids of 60 children, removed because of nasal obstruction, chronic rhinitis and recurrent respiratory infection, were evaluated. Patients were divided into two groups: group 1, suffering from chronic allergic rhinitis, and group 2, suffering from chronic rhinitis, where no specific IgE was detected, including children with a positive family history of allergy (group 2a) and children with neither a personal nor a family history of allergy (group 2b). For immunohistochemical stainings anti-CD3, anti-CD19, anti-CD4, anti-CD8, anti-CD25, anti-CD28, anti-CTLA4 (CD152), anti-bcl-2, anti-Fas, and anti-FasL antibodies were used. The number of cells expressing these molecules was identified in adenoid interfollicular spaces. The results were then analyzed in allergic and nonallergic children. During a 24-month follow-up children were re-examined for allergy and results were compared to previous immunohistochemical evaluations.
The expression of CD4, CD25, CD28, FasL, and CTLA4 was significantly increased in group 1 compared to group 2 (p < 0.05). However, the discriminant analysis confirmed that only CTLA4 and FasL expression fully discriminated allergic subjects from the others. During a 24-month period of observation 8 children from group 2a were also diagnosed with allergic rhinitis. All of them, especially those sensitized to mites, had an increased number of FasL+ and CTLA4+ in previously removed adenoids.
An increased number of cells with intracellular expression of FasL and CTLA4, in interfollicular spaces of adenoids, seems to be a predictive factor of the development of allergic rhinitis.
CD28/CTLA4衍生信号与Fas依赖性凋亡活性之间的平衡由外周防御机制决定，可能在过敏发病机制中起作用。
本研究旨在调查变应性鼻炎患儿腺样体T细胞中共刺激分子及促凋亡和抗凋亡分子的表达情况，并找出其中哪些分子在变应性鼻炎的发生发展中具有预测价值。
对60例因鼻塞、慢性鼻炎和反复呼吸道感染而切除腺样体的患儿进行评估。将患者分为两组：第1组为慢性变应性鼻炎患者，第2组为未检测到特异性IgE的慢性鼻炎患者，包括有过敏家族史的儿童（第2a组）和既无个人过敏史也无家族过敏史的儿童（第2b组）。采用抗CD3、抗CD19、抗CD4、抗CD8、抗CD25、抗CD28、抗CTLA4（CD152）、抗bcl-2、抗Fas和抗FasL抗体进行免疫组织化学染色。在腺样体滤泡间区鉴定表达这些分子的细胞数量。然后对变应性和非变应性儿童的结果进行分析。在24个月的随访期间，对儿童进行再次过敏检查，并将结果与之前的免疫组织化学评估结果进行比较。
与第2组相比，第1组中CD4、CD25、CD28、FasL和CTLA4的表达显著增加（p < 0.05）。然而，判别分析证实只有CTLA4和FasL的表达能将变应性受试者与其他受试者完全区分开来。在24个月的观察期内，第2a组的8名儿童也被诊断为变应性鼻炎。他们所有人，尤其是对螨虫致敏的儿童，在之前切除的腺样体中FasL+和CTLA4+细胞数量增加。
腺样体滤泡间区细胞内FasL和CTLA4表达增加似乎是变应性鼻炎发生发展的一个预测因素。
