School of Biological Sciences, Monash University, Melbourne, VIC, Australia.
Biotechnol Lett. 2011 Dec;33(12):2495-502. doi: 10.1007/s10529-011-0710-9. Epub 2011 Jul 24.
The gene encoding enterotoxigenic Escherichia coli B-subunit heat-labile toxin (LTB) antigen was co-transformed into hairy root cultures of Nicotiana tabacum (tobacco), Solanum lycopersicum (tomato) and Petunia parodii (petunia) under the CaMV35S promoter. Tobacco and petunia roots contained ~65-70 μg LTB g(-1) tissue whilst hairy roots of tomato contained ~10 μg LTB g(-1). Antigen at ~600 ng ml(-1) was detected in growth medium of tobacco and petunia. Tobacco roots with higher LTB levels showed growth retardation of ~80% whereas petunia hairy roots with similar levels of LTB showed only ~35% growth retardation, relative to vector controls. Regeneration of plants from LTB-containing tobacco hairy roots was readily achieved and re-initiated hairy roots from greenhouse-grown plants showed similar growth and LTB production characteristics as the original hairy root cultures.
编码肠产毒性大肠杆菌 B 亚单位不耐热肠毒素（LTB）抗原的基因在 CaMV35S 启动子的作用下被共转化到烟草、番茄和矮牵牛的发根培养物中。烟草和矮牵牛的根组织中 LTB 的含量约为 65-70 μg LTB g(-1)，而番茄的发根中 LTB 的含量约为 10 μg LTB g(-1)。在烟草和矮牵牛的生长培养基中检测到约 600 ng ml(-1)的抗原。与对照载体相比，LTB 水平较高的烟草根表现出约 80%的生长抑制，而具有相似 LTB 水平的矮牵牛发根仅表现出约 35%的生长抑制。从含有 LTB 的烟草发根中很容易再生出植物，并且从温室中生长的植物中重新诱导出的发根表现出与原始发根培养物相似的生长和 LTB 生产特性。
