DWI e.V., Institute of Technical and Macromolecular Chemistry, RWTH Aachen, D-52056 Aachen, Germany.
Macromol Biosci. 2011 Oct 10;11(10):1378-86. doi: 10.1002/mabi.201100087. Epub 2011 Jul 22.
Topographic surface patterning of intrinsically non-adhesive P(EO-stat-PO)-based hydrogels can lead to the adhesion and spreading of fibroblasts. Explanations for this unexpected behavior are discussed, particularly with regard to non-specific protein adsorption from the serum-supplemented culture medium. The presence of serum proteins is shown to be essential for adhesion. Adsorption of plasma and ECM proteins (Fibronectin (FN) and Vitronectin (VN)) to the hydrogels is possible. The effect of VN on initial cell adhesion is analyzed in detail. It appears that VN is the main serum component that is crucial for initial cell adhesion to PEG and that surface topography is essential for further, durable adhesion establishment, and spreading.
具有固有非黏附性的 P(EO-stat-PO)基水凝胶的表面形貌可以导致成纤维细胞的黏附和铺展。讨论了这种意外行为的解释，特别是关于补充有血清的培养基中非特异性蛋白质吸附的解释。结果表明，血清蛋白的存在对于黏附是必需的。可以吸附血浆和细胞外基质蛋白（纤连蛋白（FN）和玻连蛋白（VN））到水凝胶上。详细分析了 VN 对初始细胞黏附的影响。似乎 VN 是对于初始细胞黏附到 PEG 至关重要的主要血清成分，并且表面形貌对于进一步的、持久的黏附建立和铺展是必需的。
