Markina O V, Alekseeva L P, Telesmanich N R, Chemisova O S, Akulova M V, Markin N V
Klin Lab Diagn. 2011 May(5):49-52.
A new variant of enzyme immunoassay (EIA) has been developed on the basis of GM1 gangliosides to detect the toxin-producing Vibrio cholerae strains--GM1-dot-EIA. Experiments were run using a nitrocellulose membrane to bind GM1 gangliosides and polyclonal antitoxic serum to detect cholerogen. GM1-dot-EIA testing identified cholera toxin in 11 of 13 supernatants of V. cholerae eltor ctx(+) strains isolated from man and in 3 of 7 supernatants of V. cholerae eltor ctx(+) strains isolated from water. These data agree with those obtained in CM1-EIA. There was no reaction with the supernatants of other microorganisms. The sensitivity of the technique was 10 ng/ml. Thus, the simple and specific GM1-dot-EIA may be recommended to detect toxin-producing V cholerae strains isolated from man and water.
基于GM1神经节苷脂开发了一种新型酶免疫测定法（EIA），用于检测产毒素的霍乱弧菌菌株——GM1斑点酶免疫测定法。实验使用硝酸纤维素膜结合GM1神经节苷脂和多克隆抗毒素血清来检测霍乱毒素。GM1斑点酶免疫测定法检测出，从人体分离的13株埃尔托霍乱弧菌ctx(+)菌株的11份上清液以及从水中分离的7株埃尔托霍乱弧菌ctx(+)菌株的3份上清液中含有霍乱毒素。这些数据与CM1-EIA所得数据一致。与其他微生物的上清液无反应。该技术的灵敏度为10 ng/ml。因此，推荐使用简单且特异的GM1斑点酶免疫测定法来检测从人体和水中分离出的产毒素霍乱弧菌菌株。
