[用于测定霍乱弧菌培养物中霍乱毒素的酶联免疫吸附测定（ELISA）方法的评估]
[Evaluation of the ELISA method for cholera toxin determination in Vibrio cholerae cultures].
作者信息
González-Bonilla C, Gutiérrez-Cogco L, Moguel-Pech L, Villanueva-Zamudio A
机构信息
Instituto Nacional de Diagnóstico y Referencia Epidemiológicos, México, D.F., Mexico.
出版信息
Rev Latinoam Microbiol. 1994 Oct-Dec;36(4):273-6.
ELISA test was evaluated in 503 cultures of Vibrio cholerae O1 y 303 Non-O1. The cultures were isolated from sewage from different states of México between june 1991 and october 1992. The sensitivity was 100% and specificity was 96%. Only 12 strains of V. cholerae Non-O1 were positive for CT toxin. When these cultures were confirmed by polymerase chain reaction (PCR) for cholera toxin, the results were negative. ELISA test is a good alternative to be used for toxin production in cultures of V. cholerae, it needs confirmation only with O1 negative and Non-O1 positive reactions.
对503株霍乱弧菌O1和303株非O1进行了酶联免疫吸附测定（ELISA）试验。这些菌株于1991年6月至1992年10月期间从墨西哥不同州的污水中分离得到。其敏感性为100%，特异性为96%。仅12株非O1霍乱弧菌的霍乱毒素（CT）检测呈阳性。当通过聚合酶链反应（PCR）对这些培养物进行霍乱毒素确认时，结果为阴性。ELISA试验是用于检测霍乱弧菌培养物中毒素产生的一种良好替代方法，仅需对O1阴性和非O1阳性反应进行确认。
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