Gomes Rachel L, Meredith Will, Snape Colin E, Sephton Mark A
Department of Earth Science and Engineering, Imperial College London, South Kensington, London SW7 2AZ, UK.
J Pharm Biomed Anal. 2009 Jul 12;49(5):1133-40. doi: 10.1016/j.jpba.2009.01.027. Epub 2009 Jan 31.
Gas chromatography/mass spectrometry (GC/MS) is the preferred technique for the detection of urinary steroid androgens for drug testing in athletics. Excreted in either the glucuronide or sulfated conjugated form, steroids must first undergo deconjugation followed by derivatisation to render them suitable for GC analysis. Discussed herein are the deconjugation and the derivatisation preparative options. The analytical challenges surrounding these preparatory approaches, in particular the inability to cleave the sulfate moiety have led to a focus on testing protocols that reply on glucuronide conjugates. Other approaches which alleviate the need for deconjugation and derivatisation are also highlighted.
气相色谱/质谱联用（GC/MS）是体育赛事药物检测中检测尿甾体雄激素的首选技术。甾体以葡糖醛酸苷或硫酸化共轭物的形式排泄，必须首先进行去共轭，然后进行衍生化，使其适合气相色谱分析。本文讨论了去共轭和衍生化的制备方法。围绕这些制备方法的分析挑战，特别是无法裂解硫酸部分，导致人们将重点放在依赖葡糖醛酸苷共轭物的检测方案上。还强调了其他减轻去共轭和衍生化需求的方法。
