Carnovale C E, Monti J A, Catania V A, Carrillo M C
Instituto de Fisiologia Experimental, Consejo Nacional de Investigaciones Cientificas y Tecnicas, Facultad de Ciencias Bioquimicas y Farmaceuticas, Universidad Nacional de Rosario, Argentina.
Can J Physiol Pharmacol. 1990 Feb;68(2):170-3. doi: 10.1139/y90-027.
The activity of in vitro glutathione S-transferase towards 1-chloro-2,4-dinitrobenzene was examined in liver, renal cortex, and small intestine (duodenum, jejunum, ileum) after the in vivo treatment of male Wistar rats with streptozotocin or alloxan. The studies were performed at 2, 10, 24, and 48 h and 7 and 15 days after streptozotocin treatment or 24 and 48 h after alloxan treatment. The results indicated that while the blood levels of insulin-glucose did not show variations, there were no alterations of the glutathione S-transferase activity in the tissues tested. On the other hand, when the treatments caused modifications on blood insulin-glucose levels, there were changes of glutathione S-transferase activity in all tissues (except in the ileum) in such a way that a direct relationship between plasma insulin levels and glutathione S-transferase activity could be demonstrated. These results were also confirmed through insulin administration to control and diabetic rats. The data demonstrate a possible regulation of glutathione S-transferase activity by blood insulin and (or) glucose levels in the tissues tested.
在用链脲佐菌素或四氧嘧啶对雄性Wistar大鼠进行体内处理后，检测了肝脏、肾皮质和小肠（十二指肠、空肠、回肠）中体外谷胱甘肽S-转移酶对1-氯-2,4-二硝基苯的活性。研究在链脲佐菌素处理后的2、10、24和48小时以及7和15天进行，或在四氧嘧啶处理后的24和48小时进行。结果表明，虽然胰岛素-葡萄糖的血液水平没有变化，但所测试组织中的谷胱甘肽S-转移酶活性没有改变。另一方面，当处理导致血液胰岛素-葡萄糖水平发生变化时，所有组织（回肠除外）中的谷胱甘肽S-转移酶活性都发生了变化，从而可以证明血浆胰岛素水平与谷胱甘肽S-转移酶活性之间存在直接关系。通过对对照大鼠和糖尿病大鼠注射胰岛素，这些结果也得到了证实。数据表明，在所测试的组织中，谷胱甘肽S-转移酶活性可能受血液胰岛素和（或）葡萄糖水平的调节。
